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Home cDNA Clone TrueORF All POLE3 ORF Clones
TrueORF Gold

POLE3 (NM_017443) Human cDNA ORF Clone

Specifications Citations (0) Clones of Other Species Product Documents
Cat. No. Description Price Availability  
RC200874 POLE3 (Myc-DDK-tagged)-Human polymerase (DNA directed), epsilon 3 (p17 subunit) (POLE3), 10µg
$380
In Stock
TA50011-100 4C5, Anti-DDK monoclonal antibody, 100µl $248 In Stock
Clone Modification

The same insert cloned in Lenti and other vectors is also available.

Cat. No. Description Vector Price Availability  
RC200874L1 Lenti ORF clone of Human polymerase (DNA directed), epsilon 3 (p17 subunit) (POLE3) , Myc-DDK-tagged

$750 4 Weeks
TR30022 Lenti-vpak packaging kit - packaging plasmids and transfection reagent (10 rxns) Lenti-vpak-app-guide $390 In Stock
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Also for POLE3 (NM_017443)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody
TrueORF Data for RC200874
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Vector: pCMV6-Entry   Change vector? Tag: C-terminal Myc-DDK
Western validation with an anti-DDK antibody *
L: Control HEK293 lysate
R: Over-expression lysate
Lysate_Image
Sequence Data: ORF Nucleotide Sequence
Protein Sequence
ORF Size: 444 bp
Predicted Protein MW: 16.7 kDa
Restriction Sites: SgfI-MluI     Cloning Scheme for this gene     Plasmid Map Plasmid Map
OTI Annotation: This clone was engineered to express the complete ORF with an expression tag.
OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Protein Pathways: Purine metabolismPyrimidine metabolismMetabolic pathwaysDNA replicationBase excision repairNucleotide excision repair
* The lysates used for this WB picture contain the overexpressed empty vector or the Myc-DDK tagged ORF clone.

Reference Data
RefSeq: NM_017443.3, NP_059139
RefSeq Size: 2185 RefSeq ORF: 444
Synonyms : CHARAC17; CHRAC17; p17; YBL1
LocusID: 54107 Cytogenetic: 9q33 Domains: CBFD_NFYB_HMF
Summary: POLE3 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging.[supplied by OMIM, Apr 2004]. Transcript Variant: This variant (3) retains the first intron compared to variant 1. This variant is represented as non-coding due to the presence of several upstream ORFs that are predicted to interfere with translation of the longest ORF; translation of any these upstream ORFs renders the transcript a candidate for nonsense-mediated mRNA decay (NMD). ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support ERS025084, ERS025087 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

 

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