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Home cDNA Clone TrueORF All DLD ORF Clones
TrueORF Gold

DLD (NM_000108) Human cDNA ORF Clone

Specifications Citations (1) Clones of Other Species Product Documents
Cat. No. Description Price Availability  
RC200639 DLD (Myc-DDK-tagged)-Human dihydrolipoamide dehydrogenase (DLD), (10ug), 10µg
$540
In Stock
RC500639 DLD (Myc-DDK-tagged)-Human dihydrolipoamide dehydrogenase (DLD), (200ug) $660 2~3 weeks
TA50011-100 4C5, Anti-DDK monoclonal antibody, 100µl $248 In Stock
Clone Modification

The same insert cloned in Lenti and other vectors is also available.

Cat. No. Description Vector Price Availability  
RC200639L2 Lenti ORF clone of Human dihydrolipoamide dehydrogenase (DLD), mGFP tagged

$1000
$750
2 Weeks
TR30022 Lenti-vpak packaging kit - packaging plasmids and transfection reagent (10 rxns) Lenti-vpak-app-guide $390 In Stock
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Also for DLD (NM_000108)
cDNA Clone shRNA/siRNA Lysate Protein Antibody
TrueORF Data for RC200639
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Vector: pCMV6-Entry   Change vector? Tag: C-terminal Myc-DDK
Western validation with an anti-DDK antibody *
L: Control HEK293 lysate
R: Over-expression lysate
Lysate_Image
Sequence Data: ORF Nucleotide Sequence
Protein Sequence
ORF Size: 1530 bp
Predicted Protein MW: 50.1 kDa
Restriction Sites: SgfI-MluI     Cloning Scheme for this gene     Plasmid Map Plasmid Map
OTI Annotation: This clone was engineered to express the complete ORF with an expression tag.
OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Protein Families: Druggable Genome
Protein Pathways: Glycolysis / GluconeogenesisCitrate cycle (TCA cycle)Glycine, serine and threonine metabolismValine, leucine and isoleucine degradationPyruvate metabolismMetabolic pathways
* The lysates used for this WB picture contain the overexpressed empty vector or the Myc-DDK tagged ORF clone.

Reference Data
RefSeq: NM_000108.3, NP_000099
RefSeq Size: 3579 RefSeq ORF: 1530
Synonyms : DLDD; DLDH; E3; GCSL; LAD; PHE3
LocusID: 1738 Cytogenetic: 7q31 Domains: pyr_redox, pyr_redox_dim
Summary: This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In homodimeric form, the encoded protein functions as a dehydrogenase and is found in several multi-enzyme complexes that regulate energy metabolism. However, as a monomer, this protein can function as a protease. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014].

 

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