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 Home cDNA Clone TrueORF All HPRT1 ORF Clones
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HPRT1 (NM_000194) Human cDNA ORF Clone

Specifications Citations (1) Clone of Other Species Product Documents
Cat. No. Description Price Availability  
RC200462 Myc-DDK-tagged ORF clone of Homo sapiens hypoxanthine phosphoribosyltransferase 1 (HPRT1) as transfection-ready DNA, 10µg
$380
 In Stock
RC500462 Myc-DDK-tagged ORF clone of Homo sapiens hypoxanthine phosphoribosyltransferase 1 (HPRT1) as 200 ug transfection-ready DNA $500 2~3 weeks
TA50011-100 4C5, Anti-DDK monoclonal antibody, 100µl $248
$198		 In Stock

$50 off anti-DDK and other anti-tag antibodies till June 30th, 2013

The same insert cloned in Lenti and other vectors is also available.

Cat. No. Description Vector Price Availability  
RC200462L1 Lenti ORF clone of Human hypoxanthine phosphoribosyltransferase 1 (HPRT1) , Myc-DDK-tagged

$750 2 Weeks
TR30022 Lenti-vpak packaging kit - packaging plasmids and transfection reagent (10 rxns) Lenti-vpak-app-guide $360 In Stock
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Also for HPRT1 (NM_000194)
cDNA Clone shRNA/siRNA Lysate Protein Antibody
TrueORF Data for RC200462
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Vector: pCMV6-Entry   Change vector? Tag: C-terminal Myc-DDK
Western validation with an anti-DDK antibody *
L: Control HEK293 lysate
R: Over-expression lysate
Lysate_Image
Sequence Data: ORF Nucleotide Sequence
Protein Sequence 		ORF Size: 657 bp
Predicted Protein MW: 24.4 kDa
Restriction Sites: SgfI-MluI     Cloning Scheme for this gene     Plasmid Map Plasmid Map
OTI Annotation: This clone was engineered to express the complete ORF with an expression tag.
OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Protein Families: Stem cell - PluripotencyDruggable Genome
Protein Pathways: Purine metabolismDrug metabolism - other enzymesMetabolic pathways
* The lysates used for this WB picture contain the overexpressed empty vector or the Myc-DDK tagged ORF clone.

Reference Data
RefSeq: NM_000194.1, NP_000185
 RefSeq Size: 1331 RefSeq ORF: 657
Synonyms : HGPRT; HPRT
LocusID: 3251 Cytogenetic: Xq26.1 Domains: Pribosyltran
Summary: The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.[provided by RefSeq, Jun 2009].

 
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