TRAF4 (NM_004295) Human Tagged ORF Clone

CAT#: RC200345

TRAF4 (Myc-DDK-tagged)-Human TNF receptor-associated factor 4 (TRAF4)

Product Images

Circular map for RC200345

• 

  Western blot validation of overexpression lysate (Cat# LY418085) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200345 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TRAF4
• Synonyms: CART1; MLN62; RNF83
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200345 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTGGCTTCGACTACAAGTTCCTGGAGAAGCCCAAGCGACGGCTGCTGTGCCCACTGTGCGGGAAGC
  CCATGCGCGAGCCTGTGCAGGTTTCCACCTGCGGCCACCGTTTCTGCGATACCTGCCTGCAGGAGTTCCT
  CAGTGAAGGAGTCTTCAAGTGCCCTGAGGACCAGCTTCCTCTGGACTATGCCAAGATCTACCCAGACCCG
  GAGCTGGAAGTACAAGTATTGGGCCTGCCTATCCGCTGCATCCACAGTGAGGAGGGCTGCCGCTGGAGTG
  GGCCACTACGTCATCTACAGGGCCACCTGAATACCTGCAGCTTCAATGTCATTCCCTGCCCTAATCGCTG
  CCCCATGAAGCTGAGCCGCCGTGATCTACCTGCACACTTGCAGCATGACTGCCCCAAGCGGCGCCTCAAG
  TGCGAGTTTTGTGGCTGTGACTTCAGTGGGGAGGCCTATGAGAGCCATGAGGGTATGTGCCCCCAGGAGA
  GTGTCTACTGTGAGAATAAGTGTGGTGCCCGCATGATGCGGCGGCTGCTGGCCCAGCATGCCACCTCTGA
  GTGCCCCAAGCGCACTCAGCCCTGCACCTACTGCACTAAGGAGTTCGTCTTTGACACCATCCAGAGCCAC
  CAGTACCAGTGCCCAAGGCTGCCTGTTGCCTGCCCCAACCAATGTGGTGTGGGCACTGTGGCTCGGGAGG
  ACCTGCCAGGCCATCTGAAGGACAGCTGTAACACCGCCCTGGTGCTCTGCCCATTCAAAGACTCCGGCTG
  CAAGCACAGGTGCCCTAAGCTGGCAATGGCACGGCATGTGGAGGAGAGTGTGAAGCCACATCTGGCCATG
  ATGTGTGCCCTGGTGAGCCGGCAACGGCAGGAGCTGCAGGAGCTTCGGCGAGAGCTGGAGGAGCTATCAG
  TGGGCAGTGATGGCGTGCTCATCTGGAAGATTGGCAGCTATGGACGGCGGCTACAGGAGGCCAAGGCCAA
  GCCCAACCTTGAGTGCTTCAGCCCAGCCTTCTACACACATAAGTATGGTTACAAGCTGCAGGTGTCTGCA
  TTCCTCAATGGCAATGGCAGTGGTGAGGGCACACACCTCTCACTGTACATTCGTGTGCTGCCTGGTGCCT
  TTGACAATCTCCTTGAGTGGCCCTTTGCCCGCCGTGTCACCTTCTCCCTGCTGGATCAGAGCGACCCTGG
  GCTGGCTAAACCACAGCACGTCACTGAGACCTTCCACCCCGACCCAAACTGGAAGAATTTCCAGAAGCCA
  GGCACGTGGCGGGGCTCCCTGGATGAGAGTTCTCTGGGCTTTGGTTATCCCAAGTTCATCTCCCACCAGG
  ACATTCGAAAGCGAAACTATGTGCGGGATGATGCAGTCTTCATCCGTGCTGCTGTTGAACTGCCCCGGAA
  GATCCTCAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200345 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPGFDYKFLEKPKRRLLCPLCGKPMREPVQVSTCGHRFCDTCLQEFLSEGVFKCPEDQLPLDYAKIYPDP
  ELEVQVLGLPIRCIHSEEGCRWSGPLRHLQGHLNTCSFNVIPCPNRCPMKLSRRDLPAHLQHDCPKRRLK
  CEFCGCDFSGEAYESHEGMCPQESVYCENKCGARMMRRLLAQHATSECPKRTQPCTYCTKEFVFDTIQSH
  QYQCPRLPVACPNQCGVGTVAREDLPGHLKDSCNTALVLCPFKDSGCKHRCPKLAMARHVEESVKPHLAM
  MCALVSRQRQELQELRRELEELSVGSDGVLIWKIGSYGRRLQEAKAKPNLECFSPAFYTHKYGYKLQVSA
  FLNGNGSGEGTHLSLYIRVLPGAFDNLLEWPFARRVTFSLLDQSDPGLAKPQHVTETFHPDPNWKNFQKP
  GTWRGSLDESSLGFGYPKFISHQDIRKRNYVRDDAVFIRAAVELPRKILS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004295
• ORF Size: 1410 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004295.4
• RefSeq Size: 2902 bp
• RefSeq ORF: 1413 bp
• Locus ID: 9618
• UniProt ID: Q9BUZ4
• Cytogenetics: 17q11.2
• Domains: zf-TRAF, RING, MATH
• Protein Families: Druggable Genome
• Protein Pathways: Pathways in cancer, Small cell lung cancer
• MW: 53.5 kDa
• Gene Summary: This gene encodes a member of the TNF receptor associated factor (TRAF) family. TRAF proteins are associated with, and mediate signal transduction from members of the TNF receptor superfamily. The encoded protein has been shown to interact with neurotrophin receptor, p75 (NTR/NTSR1), and negatively regulate NTR induced cell death and NF-kappa B activation. This protein has been found to bind to p47phox, a cytosolic regulatory factor included in a multi-protein complex known as NAD(P)H oxidase. This protein thus, is thought to be involved in the oxidative activation of MAPK8/JNK. Alternatively spliced transcript variants have been observed but the full-length nature of only one has been determined. [provided by RefSeq, Jul 2008]