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Home cDNA Clone TrueORF All MDH1 ORF Clones
TrueORF Gold

MDH1 (NM_005917) Human cDNA ORF Clone

Specifications Citations Clones of Other Species Product Documents
Cat. No. Description Price Availability  
RC200298 MDH1 (Myc-DDK-tagged)-Human malate dehydrogenase 1, NAD (soluble) (MDH1), transcript variant 2, 10µg   
560
$98
In Stock
RG200298 MDH1 (GFP-tagged) - Human malate dehydrogenase 1, NAD (soluble) (MDH1), transcript variant 2, (10ug) $610 In Stock
TA50011-100 4C5, Anti-DDK monoclonal antibody, 100µl $248 In Stock
Cat. No. Description Price Availability
RC500298 MDH1 (Myc-DDK-tagged)-Human malate dehydrogenase 1, NAD (soluble) (MDH1), transcript variant 2, (200ug) $680 2 weeks
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Clone Modification
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The same insert cloned in Lenti and other vectors are also available.

Cat. No. Description Vector Price Availability  
RC200298L1 Lenti ORF clone of Human malate dehydrogenase 1, NAD (soluble) (MDH1), transcript variant 2 , Myc-DDK-tagged

$740 4 Weeks
TR30022 Lenti-vpak packaging kit - packaging plasmids and transfection reagent (10 rxns) Lenti-vpak-app-guide $390 In Stock
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TrueORF Data for RC200298
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Vector: pCMV6-Entry   Change vector? Tag: C-terminal Myc-DDK
Western validation with an anti-DDK antibody *
L: Control HEK293 lysate
R: Over-expression lysate
Lysate_Image
Sequence Data: ORF Nucleotide Sequence
Protein Sequence
ORF Size: 1005 bp
Predicted Protein MW: 36.2 kDa
Restriction Sites: SgfI-MluI     Cloning Scheme for this gene     Plasmid Map Plasmid Map
OTI Annotation: This clone was engineered to express the complete ORF with an expression tag.
OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Protein Families: Druggable Genome
Protein Pathways: Citrate cycle (TCA cycle)Pyruvate metabolismGlyoxylate and dicarboxylate metabolismMetabolic pathways
* The lysates used for this WB picture contain the overexpressed empty vector or the Myc-DDK tagged ORF clone.

Reference Data
RefSeq Explanation: NM_005917.2, NP_005908 RefSeq Size: 1268 RefSeq ORF: 1005
Synonyms : HEL-S-32; MDH-s; MDHA; MGC:1375; MOR2
LocusID: 4190 Cytogenetic: 2p13.3 Domains: ldh_C
Gene Summary: This gene encodes an enzyme that catalyzes the NAD/NADH-dependent, reversible oxidation of malate to oxaloacetate in many metabolic pathways, including the citric acid cycle. Two main isozymes are known to exist in eukaryotic cells: one is found in the mitochondrial matrix and the other in the cytoplasm. This gene encodes the cytosolic isozyme, which plays a key role in the malate-aspartate shuttle that allows malate to pass through the mitochondrial membrane to be transformed into oxaloacetate for further cellular processes. Alternatively spliced transcript variants have been found for this gene. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Pseudogenes have been identified on chromosomes X and 6. [provided by RefSeq, Feb 2016].

 

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