SSBP3 (NM_018070) Human Tagged ORF Clone

CAT#: RC200143

SSBP3 (Myc-DDK-tagged)-Human single stranded DNA binding protein 3 (SSBP3), transcript variant 2

Product Images

Circular map for RC200143

• 

  Western blot validation of overexpression lysate (Cat# LY413336) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200143 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SSBP3
• Synonyms: CSDP; SSDP; SSDP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200143 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTGCCAAAGGCAAAGGCTCGGCGGTGCCCTCGGATGGGCAGGCTCGGGAAAAGTTAGCTTTATACG
  TCTACGAATATTTACTGCACGTAGGAGCACAGAAATCTGCACAGACCTTCTTATCGGAGATTCGATGGGA
  AAAAAACATCACGTTGGGAGAACCGCCTGGGTTTTTGCACTCGTGGTGGTGTGTATTTTGGGACCTTTAC
  TGTGCAGCTCCTGAAAGGAGAGACACTTGTGAACATTCAAGTGAAGCAAAAGCCTTTCATGATTATAGTG
  CAGCAGCTGCCCCGAGCCCCGTGCTTGGCAACATTCCCCCCAACGATGGGATGCCGGGAGGCCCCATCCC
  GCCAGGTTTCTTTCAGGGTCCTCCGGGGTCACAGCCCTCGCCGCACGCACAGCCTCCACCTCACAATCCT
  AGCAGCATGATGGGACCCCACAGTCAGCCTCCGGGAGGAGTTCCTGGGACACAGCCATTGCTGCCCAATT
  CTATGGATCCCACACGACAACAAGGCCACCCCAACATGGGAGGATCAATGCAGAGAATGAACCCTCCCCG
  AGGCATGGGGCCCATGGGTCCCGGCCCACAGAATTACGGCAGCGGCATGAGACCACCACCCAACTCCCTC
  GGCCCCGCCATGCCCGGGATTAACATGGGCCCGGGAGCTGGCAGACCCTGGCCCAATCCTAACAGTGCTA
  ACTCAATTCCATACTCCTCCTCATCACCTGGTACCTATGTGGGACCCCCTGGTGGTGGCGGTCCTCCAGG
  AACACCCATTATGCCCAGTCCCGCAGATTCAACAAATTCCAGTGACAACATCTACACAATGATTAATCCA
  GTGCCGCCTGGAGGCAGCCGGTCCAACTTCCCGATGGGTCCCGGCTCGGACGGTCCGATGGGCGGCATGG
  GTGGCATGGAGCCACACCACATGAATGGATCATTAGGGTCAGGCGACATAGACGGACTTCCAAAAAATTC
  TCCTAACAACATAAGTGGCATTAGCAATCCTCCAGGCACCCCTCGAGATGACGGCGAGCTAGGAGGGAAC
  TTCCTCCACTCCTTTCAGAACGACAATTATTCTCCAAGCATGACGATGAGTGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200143 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MFAKGKGSAVPSDGQAREKLALYVYEYLLHVGAQKSAQTFLSEIRWEKNITLGEPPGFLHSWWCVFWDLY
  CAAPERRDTCEHSSEAKAFHDYSAAAAPSPVLGNIPPNDGMPGGPIPPGFFQGPPGSQPSPHAQPPPHNP
  SSMMGPHSQPPGGVPGTQPLLPNSMDPTRQQGHPNMGGSMQRMNPPRGMGPMGPGPQNYGSGMRPPPNSL
  GPAMPGINMGPGAGRPWPNPNSANSIPYSSSSPGTYVGPPGGGGPPGTPIMPSPADSTNSSDNIYTMINP
  VPPGGSRSNFPMGPGSDGPMGGMGGMEPHHMNGSLGSGDIDGLPKNSPNNISGISNPPGTPRDDGELGGN
  FLHSFQNDNYSPSMTMSV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018070
• ORF Size: 1104 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018070.5
• RefSeq Size: 3199 bp
• RefSeq ORF: 1107 bp
• Locus ID: 23648
• UniProt ID: Q9BWW4
• Cytogenetics: 1p32.3
• Domains: LisH, SSDP
• Protein Families: Transcription Factors
• MW: 38.2 kDa
• Gene Summary: May be involved in transcription regulation of the alpha 2(I) collagen gene where it binds to the single-stranded polypyrimidine sequences in the promoter region.[UniProtKB/Swiss-Prot Function]