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Home cDNA Clone TrueORF All NANS ORF Clones
TrueORF Gold

NANS (NM_018946) Human cDNA ORF Clone

Specifications Citations Clones of Other Species Product Documents
Cat. No. Description Price Availability  
RC200123 NANS (Myc-DDK-tagged)-Human N-acetylneuraminic acid synthase (NANS), 10µg
$540
In Stock
RC500123 NANS (Myc-DDK-tagged)-Human N-acetylneuraminic acid synthase (NANS), (200ug) $660 2~3 weeks
TA50011-100 4C5, Anti-DDK monoclonal antibody, 100µl $248 In Stock
Clone Modification

The same insert cloned in Lenti and other vectors is also available.

Cat. No. Description Vector Price Availability  
RC200123L2 Lenti ORF clone of Human N-acetylneuraminic acid synthase (NANS), mGFP tagged

1000 3 Weeks
TR30022 Lenti-vpak packaging kit - packaging plasmids and transfection reagent (10 rxns) Lenti-vpak-app-guide $390 In Stock
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Also for NANS (NM_018946)
cDNA Clone shRNA/siRNA Lysate Protein Antibody
TrueORF Data for RC200123
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Vector: pCMV6-Entry   Change vector? Tag: C-terminal Myc-DDK
Western validation with an anti-DDK antibody *
L: Control HEK293 lysate
R: Over-expression lysate
Lysate_Image
Sequence Data: ORF Nucleotide Sequence
Protein Sequence
ORF Size: 1080 bp
Predicted Protein MW: 40.1 kDa
Restriction Sites: SgfI-MluI     Cloning Scheme for this gene     Plasmid Map Plasmid Map
OTI Annotation: This clone was engineered to express the complete ORF with an expression tag.
OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Protein Pathways: Amino sugar and nucleotide sugar metabolismMetabolic pathways
* The lysates used for this WB picture contain the overexpressed empty vector or the Myc-DDK tagged ORF clone.

Reference Data
RefSeq: NM_018946.2, NP_061819
RefSeq Size: 1170 RefSeq ORF: 1080
Synonyms : HEL-S-100; SAS
LocusID: 54187 Cytogenetic: 9p24.1 Domains: NeuB, Antifreeze
Summary: This gene encodes an enzyme that functions in the biosynthetic pathways of sialic acids. In vitro, the encoded protein uses N-acetylmannosamine 6-phosphate and mannose 6-phosphate as substrates to generate phosphorylated forms of N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN), respectively; however, it exhibits much higher activity toward the Neu5Ac phosphate product. In insect cells, expression of this gene results in Neu5Ac and KDN production. This gene is related to the E. coli sialic acid synthase gene neuB, and it can partially restore sialic acid synthase activity in an E. coli neuB-negative mutant. [provided by RefSeq, Jul 2008].

 

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