GIT2 (NM_139201) Human Tagged ORF Clone

CAT#: RC200045

GIT2 (Myc-DDK-tagged)-Human G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant 4

Product Images

Circular map for RC200045

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  Western blot validation of overexpression lysate (Cat# LY408350) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200045 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified GIT2 protein (Cat# TP300045). The protein was produced from HEK293T cells transfected with GIT2 cDNA clone (Cat# RC200045) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GIT2
• Synonyms: CAT-2; CAT2; PKL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200045 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGAAACGGCTCCGGAGCAGCGAGGTGTGCGCTGACTGCAGCGGGCCGGATCCTTCCTGGGCATCAG
  TAAATAGGGGAACGTTTTTATGTGATGAGTGCTGCAGTGTCCATCGGAGTCTAGGGCGCCATATCTCCCA
  AGTGAGGCATCTGAAACACACACCGTGGCCTCCAACACTGCTTCAGATGGTTGAGACCTTGTATAATAAC
  GGTGCTAACTCTATATGGGAGCATTCTTTGCTGGACCCTGCGTCTATTATGAGTGGAAGACGTAAAGCTA
  ATCCACAGGATAAAGTACATCCCAATAAAGCGGAATTCATCAGAGCCAAGTATCAGATGTTAGCGTTCGT
  CCATCGCTTGCCCTGCCGGGATGACGATAGTGTGACTGCCAAAGATCTTAGCAAGCAACTCCATTCGAGC
  GTGAGAACAGGGAATCTTGAAACCTGTTTGAGACTGTTATCTTTAGGAGCACAAGCCAACTTCTTTCATC
  CTGAAAAAGGAAACACCCCACTCCATGTTGCCTCCAAAGCAGGGCAGATTTTACAGGCTGAATTATTGGC
  AGTATATGGAGCAGACCCAGGCACACAGGATTCTAGTGGGAAAACTCCCGTTGATTATGCAAGGCAAGGA
  GGGCACCATGAGCTGGCAGAGCGCCTCGTGGAAATACAGTATGAGCTAACGGACAGACTAGCCTTCTATC
  TCTGTGGCAGGAAACCAGATCACAAAAATGGACAGCACTTTATAATACCTCAAATGGCAGACAGCAGCCT
  GGATTTGTCTGAATTGGCAAAAGCTGCTAAGAAGAAACTTCAATCTCTAAGTAATCATTTGTTTGAAGAA
  CTTGCCATGGATATGTACGATGAAGTTGACAGGCGAGAGACGGATGCAGTCTGGCTTGCCACGCAAAACC
  ACAGCGCCCTGGTAACCGAGACAACGGTCGTCCCCTTTCTTCCGGTCAATCCTGAGTACTCATCAACACG
  AAATCAGGGCAGACAGAAGTTAGCTCGGTTCAACGCCCATGAGTTTGCCACGCTGGTCATTGACATTCTC
  AGTGACGCCAAGAGGAGACAGCAGGGCAGTTCTCTCTCGGGTTCAAAAGACAATGTGGAGCTCATACTGA
  AAACCATCAATAACCAGCACAGCGTTGAGAGTCAAGACAACGATCAGCCCGACTATGACAGCGTGGCATC
  AGACGAAGACACAGATTTGGAAACCACTGCAAGCAAAACAAACCGGCAGAAGAGCCTAGATTCAGATTTA
  TCAGATGGACCAGTCACTGTACAGGAATTTATGGAGGTCAAAAACGCTCTAGTGGCTTCTGAGGCCAAGA
  TACAGCAGCTAATGAAGGTGAATAACAACTTGAGTGACGAGCTGAGAATTATGCAGAAAAAGTTGCTTGG
  AAAAGATGCTAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200045 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSKRLRSSEVCADCSGPDPSWASVNRGTFLCDECCSVHRSLGRHISQVRHLKHTPWPPTLLQMVETLYNN
  GANSIWEHSLLDPASIMSGRRKANPQDKVHPNKAEFIRAKYQMLAFVHRLPCRDDDSVTAKDLSKQLHSS
  VRTGNLETCLRLLSLGAQANFFHPEKGNTPLHVASKAGQILQAELLAVYGADPGTQDSSGKTPVDYARQG
  GHHELAERLVEIQYELTDRLAFYLCGRKPDHKNGQHFIIPQMADSSLDLSELAKAAKKKLQSLSNHLFEE
  LAMDMYDEVDRRETDAVWLATQNHSALVTETTVVPFLPVNPEYSSTRNQGRQKLARFNAHEFATLVIDIL
  SDAKRRQQGSSLSGSKDNVELILKTINNQHSVESQDNDQPDYDSVASDEDTDLETTASKTNRQKSLDSDL
  SDGPVTVQEFMEVKNALVASEAKIQQLMKVNNNLSDELRIMQKKLLGKDAN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_139201
• ORF Size: 1413 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_139201.1, NP_631940.1
• RefSeq Size: 2357 bp
• RefSeq ORF: 1416 bp
• Locus ID: 9815
• Cytogenetics: 12q24.11
• Domains: ArfGap, ANK
• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis
• MW: 52.6 kDa
• Gene Summary: This gene encodes a member of the GIT protein family, which interact with G protein-coupled receptor kinases and possess ADP-ribosylation factor (ARF) GTPase-activating protein (GAP) activity. GIT proteins traffic between cytoplasmic complexes, focal adhesions, and the cell periphery, and interact with Pak interacting exchange factor beta (PIX) to form large oligomeric complexes that transiently recruit other proteins. GIT proteins regulate cytoskeletal dynamics and participate in receptor internalization and membrane trafficking. This gene has been shown to repress lamellipodial extension and focal adhesion turnover, and is thought to regulate cell motility. This gene undergoes extensive alternative splicing to generate multiple isoforms, but the full-length nature of some of these variants has not been determined. The various isoforms have functional differences, with respect to ARF GAP activity and to G protein-coupled receptor kinase 2 binding. [provided by RefSeq, Sep 2008]