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Home Antibody ELISA Kits

Human IL-18BP ELISA Kit

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SKU Description Manual Price Availability*  
EA100760 Human IL-18BP ELISA Kit Manual $389 2-3 Weeks Add to Shopping Cart

Specification


Product NameHuman IL-18BP ELISA Kit
Size1x96 well plate
Gene ID10068
Gene SymbolIL18BP
Assay TypeSandwich ELISA kit of Quantitative Detection for Human IL-18BP
SignalColorimetric
Format8×12 divisible strips
Assay Length3.5 hours incubations; 1 hour washing and analyzing samples
SpecificityThis kit is used for quantitative detection of human IL-18BP
ReactivityHuman
Cross ReactivityThere is no detectable cross-reactivity with other relevant proteins.
InterferenceNo significant interference observed with available related molecules.
Sample Volume100 ul
Sample TypeCell culture supernates, serum, plasma(heparin, EDTA) and urine.
Sensitivity<10pg/ml
Curve Range93.7pg/ml-6000pg/ml
BackgroundInterleukin-18-binding protein, also known as IL18BPa, is a protein that in humans is encoded by the IL18BP gene. It is mapped to 11q13.4. The protein encoded by this gene is an inhibitor of the proinflammatory cytokine IL18. This protein binds to IL18, prevents the binding of IL18 to its receptor, and thus inhibits IL18-induced IFN-gamma production. This protein is constitutively expressed and secreted in mononuclear cells. The expression of this protein can be enhanced by IFN-gamma. It has been found that IL18BP expression in intestinal tissue is increased in endothelial cells as well as cells of the submucosa and overlying lymphoid aggregates in Crohn disease.
Components
  • 96-well plate precoated with anti- human IL-18BP antibody 1
  • Lyophilized recombinant human IL-18BP standard 10ng/tubex2
  • Biotinylated anti- human IL-18BP antibody 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC) 130ul(dilution 1:100)
  • Sample diluent buffer 30 ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
Standard Curve spacer

The standard curve may vary based on the QC data of each batch. Please refer to the actual datasheet along with shipment.

 

 

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