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Home Antibody ELISA Kits

Rat GM-CSF ELISA Kit

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SKU Description Manual Price Availability*  
EA100241 Rat GM-CSF ELISA Kit Manual $389 2-3 Weeks Add to Shopping Cart

Specification


Product NameRat GM-CSF ELISA Kit
Size1x96 well plate
Gene ID116630
Gene SymbolCsf2
Assay TypeSandwich ELISA kit of Quantitative Detection for Rat GM-CSF
SignalColorimetric
Format8×12 divisible strips
Assay Length3.5 hours incubations; 1 hour washing and analyzing samples
SpecificityThis kit is used for quantitative detection of rat GM-CSF
ReactivityRat
Cross ReactivityThere is no detectable cross-reactivity with other relevant proteins.
InterferenceNo significant interference observed with available related molecules.
Sample Volume100 ul
Sample TypeCell culture supernates, serum and plasma(heparin, EDTA).
Sensitivity<10pg/ml
Curve Range15.6pg/ml-1000pg/ml
BackgroundGranulocyte-macrophage colony-stimulating factor(GM-CSF) is also symbolized CSF2. Human GM-CSF is a glycoprotein that is essential for the in vitro proliferation and differentiation of precursor cells into mature granulocytes and macrophages. The human cDNA clones contain a single open-reading frame encoding a protein of 144 amino acids with a predicted molecular mass of 16,293 daltons and show 69% nucleotide homology and 54% amino acid homology to mouse GM-CSF. The gene for human GM-CSF appears to exist as a single-copy gene. Human GM-CSF is a 22,000-dalton glycoprotein that stimulates the growth of myeloid progenitor cells and acts directly on mature neutrophils. The GM-CSF gene is localized by somatic cell hybrid analysis and in situ hybridization to human chromosome region 5q21-5q32, which is involved in interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. A complementary DNA for the T lymphocyte-derived lymphokine, GM-CSF has been cloned, and recombinant GM-CSF protein has been expressed in yeast and purified to homogeneity. This purified human recombinant GM-CSF stimulates peripheral blood monocytes in vitro to become cytotoxic for the malignant melanoma cell line A375.
Components
  • 96-well plate precoated with anti- rat GM-CSF antibody 1
  • Lyophilized recombinant rat GM-CSF standard 10ng/tubex2
  • Biotinylated anti- rat GM-CSF antibody 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100)
  • Sample diluent buffer 30ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
Standard Curve

Representative standard curve for Rat GM-CSF PicoKine ELISA Kit

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The standard curve may vary based on the QC data of each batch. Please refer to the actual datasheet along with shipment.

 

 

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