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Home Antibody ELISA Kits

Rat IL-2 ELISA Kit (96-well)

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SKU Description Manual Price Availability*  
EA100160 Rat IL-2 ELISA Kit (96-well) Manual $400 2-3 Weeks Add to Shopping Cart

Specification


Product NameRat IL-2 ELISA Kit (96-well)
Size96 wells
Gene ID116562
Gene SymbolIl2
Assay TypeSolid Phase Sandwich ELISA
SignalColorimetric
Format96-well strip plate
Assay Length3 hour
SpecificityNatural and recombinant Rat IL-2 Ligand
ReactivityRat
Cross Reactivity
InterferenceNo significant interference observed with available related molecules.
Sample Volume20 uL
Sample TypeCell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sensitivity7pg/mL
Curve Range0.03 - 2.0 ng/mL
Background

Interleukin 2 (IL-2) is a pleiotropic cytokine produced primarily by mitogen- or antigen-activated T lymphocytes . It plays a key role in promoting the clonal expansion of antigen-specific T cells. In addition, IL-2 has been shown to mediate multiple immune responses on a variety of cell types.

IL-2 has been found to stimulate growth and differentiation of B cells, NK cells, LAK cells, monocytes, and oligodendocytes. IL-2 is involved in treatment of cancers such as melanoma and renal cell cancer. It plays a key role in promoting the clonal expansion of antigen-specific T cells. In addition, IL-2 has also been shown to mediate multiple immune responses on a variety of cell types.

The sequence of rat IL-2 cDNA predicts a 155 amino acid (aa) residue precursor glycoprotein containing a 20 aa residue signal peptide that is cleaved to form the mature protein . At the amino acid sequence level, mature rat IL-2 is approximately 80% identical to mouse IL-2 .

The biological effects of IL-2 are mediated by specific cell surface receptor complexes. The functional high-affinity receptor for IL-2 is composed of three distinct polypeptide chains.

IL-2 stimulates the proliferation of thymocytes; stimulates the proliferation and differentiation of activated B cells; promotes the growth, differentiation and cytocidal activity of monocytes; induces the growth of and cytokine production and cytolytic activity by natural killer cells; enhances the production of lymphocyte-activated killer (LAK) cells; and induces the proliferation and differentiation of oligodendrocytes .

Components
  • Aluminium pouches with a Microwell Plate coated with monoclonal antibody to rat IL-2 (8X12)
  • 2 vials rat IL-2 Standard lyophilized,4000 pg/ml upon reconstitution
  • 2 vials concentrated Biotin-Conjugate anti-rat IL-2 antibody
  • 2 vials Streptavidin-HRP solution,
  • 1 bottle Standard /sample Diluent
  • 1 bottle Biotin-Conjugate antibody Diluent
  • 1 bottle Streptavidin-HRP Diluent
  • 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  • 1 vial Substrate Solution
  • 1 vial Stop Solution
  • 3 pieces Adhesive Films
  • package insert
Standard Curve

Representative standard curve for IL-2 ELISA. IL-2 was diluted in serial two-fold steps in Sample Diluent.

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The standard curve may vary based on the QC data of each batch. Please refer to the actual datasheet along with shipment.

 

 

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