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HS578T (Human Breast Carcinoma) Whole Cell Lysate

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SKU Description Amount Price Availability
LY500022 HS578T (Human Breast Carcinoma) Whole Cell Lysate 100ul/100ug $120 1 week
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Product Specification

TissueBreast
HostHuman
Lysis BufferModified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma) and 1mM Na3VO4)
Component1 vial of 100ug whole cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromphenol blue, 100mM DTT)
StorageThe lysate is shipped with dry ice. Upon receiving, store the sample at -20ºC. Lysate samples are stable for 12 months from date of receipt when stored at -20ºC. Avoid repeated freeze-thaw cycles. 
Lysate samples can be diluted with 2xSDS Sample Buffer provided. After dilution, the protein sample should be aliquoted and stored at -80ºC for long term storage. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Recommended ApplicationWB, IP

 

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