Synthetic single guide (sgRNA) for efficient genome editing
Pure and simple
CRISPR application requires 2 key functional elements: target-specific guide RNA and CAS9 enzyme. Scientists have created gRNA using multiple methods, such as transfection of gRNA-expression plasmids, in vitro transcription, or by annealing a short crRNA oligo with a tracrRNA scaffold.
Recently, synthetic single guide has been recognized as the preferred way for highly efficient and accurate editing. The synthetic single gRNA is a pure 100-mer RNA oligo that contains the target gRNA sequence and the tracrRNA scaffold in a single entity.
The editing efficiency (solid bars) and consistency (error lines) of synthetic sgRNAs against in vitro transcribed (IVT) guide RNAs in HEK293T cells. The experiment was conducted by a third-party using three different gene targets and was replicated three times.
Benefits of synthetic sgRNA (100mer):
Simple workflow Ready-to-use solution No in vitro transcription (as in IVT) No annealing/purification (as in the 2-oligo system)
Better in vivo stability No worry for RNase carry-over from the source
100% DNA-free No risk of integrating foreign DNA into cell line
Better efficiency Up to 90% genome editing efficiency
Cost-effective Highly scalable for large numbers of experiments
Fast delivery sgRNA shipped out in 5-7 days
How can sgRNA be used?
Transfection of sgRNA into a CAS9-expression cell line
Transfection of RNP of sgRNA/CAS9 protein into target cells
Transfection of sgRNA and a CAS9-expression plasmid into the target cells
Transfection of sgRNA and CAS9 mRNA into the target cells
Microinjection of sgRNA (together with Cas9 protein or mRNA) into embryo/cells
What will we deliver?
Synthetic CRISPR sgRNA (1nmol)
Sufficient for 10-20 Transfections
QC through Mass Spectrometry
How to order
Click on the quote button and input a 17-20nt target sequence for each sgRNA
OriGene recommends 3-5 sgRNA per target gene.
Place order via email or phone call (1.888.267.4436)
Contact us for modified RNA option or volume discount