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Home CRISPR-CAS9 KN313886

Prkch - mouse gene knockout kit via CRISPR

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Specifications  Citations  Validation Data  FAQ
SKU Description Price Availability Manual  
KN313886 Prkch - mouse gene knockout kit via CRISPR $1200 4 weeks Manual PDF Add to Shopping Cart
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Also for Prkch (Locus ID 18755)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Request Antibody
SKU Description Donor Vector Price Availability  
KN313886RB Prkch - mouse gene knockout kit via CRISPR RFP-BSD 1290 4 weeks
KN313886LP Prkch - mouse gene knockout kit via CRISPR Luciferase-Puro 1290 4 Weeks
KN313886BN Prkch - mouse gene knockout kit via CRISPR mBFP-Neo 1290 4 Weeks
Add to Shopping Cart

Comparing to KN313886, the above kits contain:

  • Identical gRNA vectors
  • Identical LHA & RHA
  • Different donor cassette
kn_donor_diagram
Kit Components
KN313886G1, Prkch gRNA vector 1 in pCas-Guide vector
KN313886G2, Prkch gRNA vector 2 in pCas-Guide vector
KN313886D, donor vector containing Left and right homologous arms and GFP-Puro functional cassette.
   
GE100003, scramble sequence in pCas-Guide vector

Disclaimer
The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data
RefSeq: BC031121NM_001313977NM_008856
Synonyms:
Summary: Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipids-dependent protein kinase. It is predominantly expressed in epithelial tissues and has been shown to reside specifically in the cell nucleus. This protein kinase can regulate keratinocyte differentiation by activating the MAP kinase MAPK13 (p38delta)-activated protein kinase cascade that targets CCAAT/enhancer-binding protein alpha (CEBPA). It is also found to mediate the transcription activation of the transglutaminase 1 (TGM1) gene. Mutations in the human gene are associated with susceptibility to cerebral infarction. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015].

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Diagram-Gene Editing RecombII


 

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