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Home CRISPR-CAS9 KN313871

Prkaca - mouse gene knockout kit via CRISPR

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Specifications  Citations  Validation Data  FAQ
SKU Description Price Availability Manual  
KN313871 Prkaca - mouse gene knockout kit via CRISPR $1200 4 weeks Manual PDF Add to Shopping Cart
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Also for Prkaca (Locus ID 18747)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Request Antibody
SKU Description Donor Vector Price Availability  
KN313871RB Prkaca - mouse gene knockout kit via CRISPR RFP-BSD 1290 4 weeks
KN313871LP Prkaca - mouse gene knockout kit via CRISPR Luciferase-Puro 1290 4 Weeks
KN313871BN Prkaca - mouse gene knockout kit via CRISPR mBFP-Neo 1290 4 Weeks
Add to Shopping Cart

Comparing to KN313871, the above kits contain:

  • Identical gRNA vectors
  • Identical LHA & RHA
  • Different donor cassette
kn_donor_diagram
Kit Components
KN313871G1, Prkaca gRNA vector 1 in pCas-Guide vector
KN313871G2, Prkaca gRNA vector 2 in pCas-Guide vector
KN313871D, donor vector containing Left and right homologous arms and GFP-Puro functional cassette.
   
GE100003, scramble sequence in pCas-Guide vector

Disclaimer
The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data
RefSeq: BC003238BC054834NM_001277898NM_008854
Synonyms: Cs, PKA, PKCD
Summary: This gene encodes a member of the serine/threonine protein kinase family. The holoenzyme, protein kinase A (also known as cyclic-AMP dependent protein kinase), mediates cellular response to changes in cyclic-AMP levels. This gene encodes the alpha catalytic subunit of protein kinase A. Protein kinase A-mediated signaling is transduced via phosphorylation of target proteins, and is important for many cellular functions, including mammalian sperm maturation and motility. Alternative splicing results in multiple transcript variants. A pseudogene of this gene has been defined on the X chromosome. [provided by RefSeq, Apr 2013].

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Diagram-Gene Editing RecombII


 

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