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Home CRISPR-CAS9 KN213409

CTSA - human gene knockout kit via CRISPR

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Specifications  Citations  Validation Data  FAQ
SKU Description Price Availability Manual  
KN213409 CTSA - human gene knockout kit via CRISPR $1200 4 Weeks Manual PDF Add to Shopping Cart
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SKU Description Donor Vector Price Availability  
KN213409RB CTSA - human gene knockout kit via CRISPR RFP-BSD 1290 4 Weeks
KN213409LP CTSA - human gene knockout kit via CRISPR Luciferase-Puro 1290 4 Weeks
KN213409BN CTSA - human gene knockout kit via CRISPR mBFP-Neo 1290 4 Weeks
Add to Shopping Cart

Comparing to KN213409, the above kits contain:

  • Identical gRNA vectors
  • Identical LHA & RHA
  • Different donor cassette
kn_donor_diagram
Kit Components
KN213409G1, CTSA gRNA vector 1 in pCas-Guide vector
KN213409G2, CTSA gRNA vector 2 in pCas-Guide vector
KN213409D, donor vector containing Left and right homologous arms and GFP-Puro functional cassette.
   
GE100003, scramble sequence in pCas-Guide vector

Disclaimer
The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data
RefSeq: NM_000308NM_001127695NM_001167594
Synonyms: GLB2; GSL; NGBE; PPCA; PPGB
Summary: This gene encodes a member of the peptidase S10 family of serine carboxypeptidases. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate two chains that comprise the heterodimeric active enzyme. This enzyme possesses deamidase, esterase and carboxypeptidase activities and acts as a scaffold in the lysosomal multienzyme complex. Mutations in this gene are associated with galactosialidosis. [provided by RefSeq, Nov 2015]

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Diagram-Gene Editing RecombII


 

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