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Home CRISPR-CAS9 KN209465

COL18A1 - human gene knockout kit via CRISPR

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Specifications  Citations  Validation Data  FAQ
SKU Description Price Availability Manual  
KN209465 COL18A1 - human gene knockout kit via CRISPR $1200 7 Days * Manual PDF Add to Shopping Cart

* Business Days

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Also for COL18A1 (Locus ID 80781)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Request Antibody
SKU Description Donor Vector Price Availability  
KN209465RB COL18A1 - human gene knockout kit via CRISPR RFP-BSD 1290 7 Days
KN209465LP COL18A1 - human gene knockout kit via CRISPR Luciferase-Puro 1290 4 Weeks
KN209465BN COL18A1 - human gene knockout kit via CRISPR mBFP-Neo 1290 4 Weeks
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Comparing to KN209465, the above kits contain:

  • Identical gRNA vectors
  • Identical LHA & RHA
  • Different donor cassette
kn_donor_diagram
Kit Components
KN209465G1, COL18A1 gRNA vector 1 in pCas-Guide vector, Target Sequence: CGACCCCCGCAGCCGCATCT
KN209465G2, COL18A1 gRNA vector 2 in pCas-Guide vector, Target Sequence: GGTTGGGGGACGCCAAGATG
KN209465D, donor vector containing Left and right homologous arms and GFP-Puro functional cassette.
Homologous arm and GFP-puro sequences   
GE100003, scramble sequence in pCas-Guide vector

Disclaimer
The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data
RefSeq: NM_030582NM_130444NM_130445
Synonyms: KNO; KNO1; KS
Summary: Endostatin potently inhibits endothelial cell proliferation and angiogenesis. May inhibit angiogenesis by binding to the heparan sulfate proteoglycans involved in growth factor signaling. [UniProtKB/Swiss-Prot Function]

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Diagram-Gene Editing RecombII


 

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