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Home CRISPR-CAS9 KN207496

EEF2K - human gene knockout kit via CRISPR

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Specifications  Citations  Validation Data  FAQ
SKU Description Price Availability Manual  
KN207496 EEF2K - human gene knockout kit via CRISPR $1200 4 Weeks Manual PDF Add to Shopping Cart
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Also for EEF2K (Locus ID 29904)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Request Antibody
SKU Description Donor Vector Price Availability  
KN207496RB EEF2K - human gene knockout kit via CRISPR RFP-BSD 1290 4 Weeks
KN207496LP EEF2K - human gene knockout kit via CRISPR Luciferase-Puro 1290 4 Weeks
KN207496BN EEF2K - human gene knockout kit via CRISPR mBFP-Neo 1290 4 Weeks
Add to Shopping Cart

Comparing to KN207496, the above kits contain:

  • Identical gRNA vectors
  • Identical LHA & RHA
  • Different donor cassette
kn_donor_diagram
Kit Components
KN207496G1, EEF2K gRNA vector 1 in pCas-Guide vector
KN207496G2, EEF2K gRNA vector 2 in pCas-Guide vector
KN207496D, donor vector containing Left and right homologous arms and GFP-Puro functional cassette.
   
GE100003, scramble sequence in pCas-Guide vector

Disclaimer
The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data
RefSeq: NM_013302XM_017023193XM_017023194XM_171968XR_001751897XR_950786XR_950787
Synonyms: eEF-2K; HSU93850
Summary: This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment. [provided by RefSeq, Jul 2008].

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Diagram-Gene Editing RecombII


 

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