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Home CRISPR-CAS9 KN206533

CHST4 - human gene knockout kit via CRISPR

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Specifications  Citations  Validation Data  FAQ
SKU Description Price Availability Manual  
KN206533 CHST4 - human gene knockout kit via CRISPR $1200 4 Weeks Manual PDF Add to Shopping Cart
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Also for CHST4 (Locus ID 10164)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Request Antibody
SKU Description Donor Vector Price Availability  
KN206533RB CHST4 - human gene knockout kit via CRISPR RFP-BSD 1290 4 Weeks
KN206533LP CHST4 - human gene knockout kit via CRISPR Luciferase-Puro 1290 4 Weeks
KN206533BN CHST4 - human gene knockout kit via CRISPR mBFP-Neo 1290 4 Weeks
Add to Shopping Cart

Comparing to KN206533, the above kits contain:

  • Identical gRNA vectors
  • Identical LHA & RHA
  • Different donor cassette
kn_donor_diagram
Kit Components
KN206533G1, CHST4 gRNA vector 1 in pCas-Guide vector
KN206533G2, CHST4 gRNA vector 2 in pCas-Guide vector
KN206533D, donor vector containing Left and right homologous arms and GFP-Puro functional cassette.
   
GE100003, scramble sequence in pCas-Guide vector

Disclaimer
The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data
RefSeq: NM_001166395NM_005769
Synonyms: GlcNAc6ST2; GST3; HECGLCNAC6ST; LSST
Summary: This gene encodes an N-acetylglucosamine 6-O sulfotransferase. The encoded enzyme transfers sulfate from 3'phosphoadenosine 5'phospho-sulfate to the 6-hydroxyl group of N-acetylglucosamine on glycoproteins. This protein is localized to the Golgi and is involved in the modification of glycan structures on ligands of the lymphocyte homing receptor L-selectin. Alternate splicing in the 5' UTR results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2009].

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Diagram-Gene Editing RecombII


 

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