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Home CRISPR-CAS9 KN205240

SPAG8 - human gene knockout kit via CRISPR

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Specifications  Citations  Validation Data  FAQ
SKU Description Price Availability Manual  
KN205240 SPAG8 - human gene knockout kit via CRISPR $1200 4 Weeks Manual PDF Add to Shopping Cart
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Also for SPAG8 (Locus ID 26206)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Request Antibody
SKU Description Donor Vector Price Availability  
KN205240RB SPAG8 - human gene knockout kit via CRISPR RFP-BSD 1290 4 Weeks
KN205240LP SPAG8 - human gene knockout kit via CRISPR Luciferase-Puro 1290 4 Weeks
KN205240BN SPAG8 - human gene knockout kit via CRISPR mBFP-Neo 1290 4 Weeks
Add to Shopping Cart

Comparing to KN205240, the above kits contain:

  • Identical gRNA vectors
  • Identical LHA & RHA
  • Different donor cassette
kn_donor_diagram
Kit Components
KN205240G1, SPAG8 gRNA vector 1 in pCas-Guide vector
KN205240G2, SPAG8 gRNA vector 2 in pCas-Guide vector
KN205240D, donor vector containing Left and right homologous arms and GFP-Puro functional cassette.
   
GE100003, scramble sequence in pCas-Guide vector

Disclaimer
The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data
RefSeq: BC019247NM_001039592NM_012436NM_172312
Synonyms: BS-84; CILD28; CT142; HSD-1; hSMP-1; SMP1; SPAG3
Summary: The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein encoded by this gene is recognized by sperm agglutinating antibodies from an infertile woman. This protein is localized in germ cells of the testis at all stages of spermatogenesis and is localized to the acrosomal region of mature spermatozoa. This protein interacts with ACT (activator of CREM in testis) and may play a role in CREM (cAMP response element modulator)-ACT-mediated gene transcription during spermatogenesis. This protein may also play a role in spermatogenesis by regulating microtubule formation and cell division. Alternatively spliced variants that encode different protein isoforms have been described but the full-length sequences of only two have been determined. [provided by RefSeq, Jul 2012].

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Diagram-Gene Editing RecombII


 

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