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Home CRISPR-CAS9 KN204804

BCAP31 - human gene knockout kit via CRISPR

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Specifications  Citations  Validation Data  FAQ
SKU Description Price Availability Manual  
KN204804 BCAP31 - human gene knockout kit via CRISPR $1200 4 Weeks Manual PDF Add to Shopping Cart
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Also for BCAP31 (Locus ID 10134)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Request Antibody
SKU Description Donor Vector Price Availability  
KN204804RB BCAP31 - human gene knockout kit via CRISPR RFP-BSD 1290 4 Weeks
KN204804LP BCAP31 - human gene knockout kit via CRISPR Luciferase-Puro 1290 4 Weeks
KN204804BN BCAP31 - human gene knockout kit via CRISPR mBFP-Neo 1290 4 Weeks
Add to Shopping Cart

Comparing to KN204804, the above kits contain:

  • Identical gRNA vectors
  • Identical LHA & RHA
  • Different donor cassette
kn_donor_diagram
Kit Components
KN204804G1, BCAP31 gRNA vector 1 in pCas-Guide vector
KN204804G2, BCAP31 gRNA vector 2 in pCas-Guide vector
KN204804D, donor vector containing Left and right homologous arms and GFP-Puro functional cassette.
   
GE100003, scramble sequence in pCas-Guide vector

Disclaimer
The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data
RefSeq: NM_001139441NM_001139457NM_001256447NM_005745NR_024450
Synonyms: 6C6-AG; BAP31; CDM; DDCH; DXS1357E
Summary: This gene encodes a member of the B-cell receptor associated protein 31 superfamily. The encoded protein is a multi-pass transmembrane protein of the endoplasmic reticulum that is involved in the anterograde transport of membrane proteins from the endoplasmic reticulum to the Golgi and in caspase 8-mediated apoptosis. Microdeletions in this gene are associated with contiguous ABCD1/DXS1375E deletion syndrome (CADDS), a neonatal disorder. Alternative splicing of this gene results in multiple transcript variants. Two related pseudogenes have been identified on chromosome 16. [provided by RefSeq, Jan 2012].

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Diagram-Gene Editing RecombII


 

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