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Home CRISPR-CAS9 KN202677

GCA - human gene knockout kit via CRISPR

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Specifications  Citations  Validation Data  FAQ
SKU Description Price Availability Manual  
KN202677 GCA - human gene knockout kit via CRISPR $1200 4 Weeks Manual PDF Add to Shopping Cart
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SKU Description Donor Vector Price Availability  
KN202677RB GCA - human gene knockout kit via CRISPR RFP-BSD 1290 4 Weeks
KN202677LP GCA - human gene knockout kit via CRISPR Luciferase-Puro 1290 4 Weeks
KN202677BN GCA - human gene knockout kit via CRISPR mBFP-Neo 1290 4 Weeks
Add to Shopping Cart

Comparing to KN202677, the above kits contain:

  • Identical gRNA vectors
  • Identical LHA & RHA
  • Different donor cassette
kn_donor_diagram
Kit Components
KN202677G1, GCA gRNA vector 1 in pCas-Guide vector
KN202677G2, GCA gRNA vector 2 in pCas-Guide vector
KN202677D, donor vector containing Left and right homologous arms and GFP-Puro functional cassette.
   
GE100003, scramble sequence in pCas-Guide vector

Disclaimer
The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data
RefSeq: NM_001330265NM_001330266NM_001330267NM_001330268NM_001330270NM_001330271NM_012198XM_005246446XM_006712398XM_006712400XM_011510926XM_011510928XM_017003767XM_017003769
Synonyms: GCL; GCL
Summary: This gene encodes a calcium-binding protein that is abundant in neutrophils and macrophages. In the absence of divalent cation, this protein localizes to the cytosolic fraction; with magnesium alone, it partitions with the granule fraction; and in the presence of magnesium and calcium, it associates with both the granule and membrane fractions. Alternative splicing and use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Aug 2016].

Learn more about CRISPR?

Diagram-Gene Editing RecombII


 

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