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Home CRISPR-CAS9 KN200879

PUS1 - human gene knockout kit via CRISPR

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Specifications  Citations  Validation Data  FAQ
SKU Description Price Availability Manual  
KN200879 PUS1 - human gene knockout kit via CRISPR $1200 4 Weeks Manual PDF Add to Shopping Cart
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SKU Description Donor Vector Price Availability  
KN200879RB PUS1 - human gene knockout kit via CRISPR RFP-BSD 1290 4 Weeks
KN200879LP PUS1 - human gene knockout kit via CRISPR Luciferase-Puro 1290 4 Weeks
KN200879BN PUS1 - human gene knockout kit via CRISPR mBFP-Neo 1290 4 Weeks
Add to Shopping Cart

Comparing to KN200879, the above kits contain:

  • Identical gRNA vectors
  • Identical LHA & RHA
  • Different donor cassette
kn_donor_diagram
Kit Components
KN200879G1, PUS1 gRNA vector 1 in pCas-Guide vector
KN200879G2, PUS1 gRNA vector 2 in pCas-Guide vector
KN200879D, donor vector containing Left and right homologous arms and GFP-Puro functional cassette.
   
GE100003, scramble sequence in pCas-Guide vector

Disclaimer
The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data
RefSeq: NM_001002019NM_001002020NM_025215XM_011538768XM_011538769XR_001748872
Synonyms: MLASA1
Summary: This gene encodes a pseudouridine synthase that converts uridine to pseudouridine once it has been incorporated into an RNA molecule. The encoded enzyme may play an essential role in tRNA function and in stabilizing the secondary and tertiary structure of many RNAs. A mutation in this gene has been linked to mitochondrial myopathy and sideroblastic anemia. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Sep 2009].

Learn more about CRISPR?

Diagram-Gene Editing RecombII


 

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