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Home CRISPR-CAS9 KN200855

POLR2F - human gene knockout kit via CRISPR

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Specifications  Citations  Validation Data  FAQ
SKU Description Price Availability Manual  
KN200855 POLR2F - human gene knockout kit via CRISPR $1200 4 Weeks Manual PDF Add to Shopping Cart
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Also for POLR2F (Locus ID 5435)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Request Antibody
SKU Description Donor Vector Price Availability  
KN200855RB POLR2F - human gene knockout kit via CRISPR RFP-BSD 1290 4 Weeks
KN200855LP POLR2F - human gene knockout kit via CRISPR Luciferase-Puro 1290 4 Weeks
KN200855BN POLR2F - human gene knockout kit via CRISPR mBFP-Neo 1290 4 Weeks
Add to Shopping Cart

Comparing to KN200855, the above kits contain:

  • Identical gRNA vectors
  • Identical LHA & RHA
  • Different donor cassette
kn_donor_diagram
Kit Components
KN200855G1, POLR2F gRNA vector 1 in pCas-Guide vector
KN200855G2, POLR2F gRNA vector 2 in pCas-Guide vector
KN200855D, donor vector containing Left and right homologous arms and GFP-Puro functional cassette.
   
GE100003, scramble sequence in pCas-Guide vector

Disclaimer
The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data
RefSeq: NM_001301129NM_001301130NM_001301131NM_021974NR_125371XM_017028832
Synonyms: HRBP14.4; POLRF; RPABC14.4; RPABC2; RPB14.4; RPB6; RPC15
Summary: This gene encodes the sixth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. In yeast, this polymerase subunit, in combination with at least two other subunits, forms a structure that stabilizes the transcribing polymerase on the DNA template. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014].

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Diagram-Gene Editing RecombII


 

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