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Home CRISPR-CAS9 KN200376

BUB3 - human gene knockout kit via CRISPR

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Specifications  Citations  Validation Data  FAQ
SKU Description Price Availability Manual  
KN200376 BUB3 - human gene knockout kit via CRISPR $1200 4 Weeks Manual PDF Add to Shopping Cart
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SKU Description Donor Vector Price Availability  
KN200376RB BUB3 - human gene knockout kit via CRISPR RFP-BSD 1290 4 Weeks
KN200376LP BUB3 - human gene knockout kit via CRISPR Luciferase-Puro 1290 4 Weeks
KN200376BN BUB3 - human gene knockout kit via CRISPR mBFP-Neo 1290 4 Weeks
Add to Shopping Cart

Comparing to KN200376, the above kits contain:

  • Identical gRNA vectors
  • Identical LHA & RHA
  • Different donor cassette
kn_donor_diagram
Kit Components
KN200376G1, BUB3 gRNA vector 1 in pCas-Guide vector
KN200376G2, BUB3 gRNA vector 2 in pCas-Guide vector
KN200376D, donor vector containing Left and right homologous arms and GFP-Puro functional cassette.
   
GE100003, scramble sequence in pCas-Guide vector

Disclaimer
The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data
RefSeq: NM_001007793NM_004725
Synonyms: BUB3L; hBUB3
Summary: This gene encodes a protein involved in spindle checkpoint function. The encoded protein contains four WD repeat domains and has sequence similarity with the yeast BUB3 protein. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008].

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Diagram-Gene Editing RecombII


 

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