EP300 Human Gene Knockout Kit (CRISPR)

CAT#: KN223265

EP300 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: EP300
• Locus ID: 2033
• Components:

  KN223265G1, EP300 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: ATCGCTGGCGGACGCCGAGA

  KN223265G2, EP300 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: AGATGAGAGTTTAGGCCGCT

  KN223265D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  CTCTCGGCTC GGGCGAGTTC TCTGCGGCCA TTAGGGGCCG GTGCGGCGGC GGCGGCGCGG AGCGCGGCGG CAGGAGGAGG GTTCGGAGGG TGGGGGCGCA GGCCCGGGAG GGGGCACCGG GAGGAGGTGA GTGTCTCTTG TCGCCTCCTC CTCTCCCCCC TTTTCGCCCC CGCCTCCTTG TGGCGATGAG AAGGAGGAGG ACAGCGCCGA GGAGGAAGAG GTTGATGGCG GCGGCGGAGC TCCGAGAGAC CTCGGCTGGG CAGGGGCCGG CCGTGGCGGG CCGGGGACTG CGCCTCTAGA GCCGCGAGTT CTCGGGAATT CGCCGCAGCG GACGCGCTCG GCGAATTTGT GCTCTTGTGC CCTCCTCCGG GCTTGGGCCC AGGCCCGGCC CCTCGCACTT GCCCTTACCT TTTCTATCGA GTCCGCATCC CTCTCCAGCC ACTGCGACCC GGCGAAGAGA AAAAGGAACT TCCCCCACCC CCTCGGGTGC CGTCGGAGCC CCCCAGCCCA CCCCTGGGTG CGGCGCGGGG ACCCCGGGCC GAAGAAGAGA TTTCCTGAGG ATTCTGGTTT TCCTCGCTTG TATCTCCGAA AGAATTAAAA GGCACAGGTT AGTTTCGGCA GCCCCGGCCT TCCACGTTCC CTTTAATCTT TTCTACTCGG TGCGCCTTTA TTCTTCCATT TTTTTTTTCT TCCTCTCTCT CTAGTTCCCT GCCCCTTAAT TAATTTTAAA GGTATTTGAA TGAGCTGGTC GAAGACGTCC AGTAGCCCAA CCATTTTCTT TGCCTCCTAA TACATTGATT GCAACACTAT GTCATATCGG TGTGTGTTCT GGAATTAGAG CTCGTAAGTG GGTGCTATAT AGAAATGGCC TGTATTGTTG CTCTCATGCA ATTTAATTTG GGAAATGCCA ATGCATTTGT GTGTAAGAGC TCCTATGCAA TTTAAGTTTC ATTTTTTTTG TTCTTGGCAA ATGAATTTCG AATCCTTTCT CTTTACTGTA CTTACTGTGT TTCCTCTACT TTCCACTCTT CGAAATTTTC TCTCCTTTGC AGAATATTTT CTGTTGAGGA ATAGGGTGTA AAAAAATCTT GATTTTGTGT GTGCGAGGTC CAAGATTTCA GGTTAGAGGT GAAGTTTAAC AATTCTTTTA TCTTACCTAC CATTCTTAGC TTTTTTTTCC TTTTTCTTCT TATTTGCTTT

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001429, N57495, NM_001362843
• UniProt ID: Q09472
• Synonyms: KAT3B; p300; RSTS2
• Summary: This gene encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. It functions as histone acetyltransferase that regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. It mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. This gene has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Defects in this gene are a cause of Rubinstein-Taybi syndrome and may also play a role in epithelial cancer. [provided by RefSeq, Jul 2008]