PFKFB3 Human Gene Knockout Kit (CRISPR)

CAT#: KN206043

PFKFB3 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: PFKFB3
• Locus ID: 5209
• Components:

  KN206043G1, PFKFB3 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GCTGGACACGTTTAGTCCCA

  KN206043G2, PFKFB3 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: GTGGCAGAGCCCCTGCTTGC

  KN206043D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  CCTGCAGGGG CTGCCCTCCG CCTTTTCCGC CCTGCGTGCC AGAAAGGCCC GGCTACCTGG TGTTCCTACC CGCACCGGGG GGTCCCAGCG CACCGGACCC CGGTTTACAG GCCGGGTGGT TGGAGGCCCA GCACTGCCCA GAGCCCTGGG CCCCTCTCAG CTCAAAGACC CGGGAGAGAA GTGGGAGCTG CAGAAGTGTG GGGAGGGGGG CCTCGATTTG ACTTTCAAAT CAGAGTTGTG TGACTCCTGC GCCGTCTGTT TGGGTTGGGA GGGGTTGAGT TCTGGTGATG ATTGTCACTC AACACCCGAG ACTCCCCCAC TGCGACCTGG GCACACTAAG GACGTTGAGG ACTGGGGGTG TGTGTGGGGG GAGCCTGGCC TCCCCTGGCA CTGGGCCTGT GCTGTGCCGT CTCTCCCTTC CCGCACCCAC CCATCTGCCT CTCTTCTCTC ATAACTCAGA GGCACGGCCA GCGTGATGCT ACGGTTGCCT GGAGGCTGTA CCGGACTTGT TTTTCAAACA GAGGGTGCCT AGGTATCAGC GTGGGCTCCT GGCGGTGAAG GGGGTGGCTG CTGACTCTCC TGTCCCGTTG GGGTAGAGAG TCGGCCACCT TGACTCTGTG GGTTCTCTGG AGGCTCTCAG AGTGTCCCTC CCCCCCTACT CATTAACTGC AGGGGACAAT CATTTATCTC TGACTTCATC GCTTCTGCCC ACTTTTGTCG ATGTAGGCTC TGGTTGGGAA ACTCCTAAAA GGGACAGACT TCTGTTTACT TGTGCGCTCA GTAGAGAGCC TTATGCGGCA AAGTATTAAA TAGCCAAGCT GCTAATGTAT TGTTTGCATA TGAAGATTTT GCTTTATGAC AGCGTATTTT AGTGCATTAT TAGATCACAC AGGACAAAAC ATTTGCAAAC TGCCAACCAA GGTAAGCTTT GGTTTTTTGG TTATTATCAT TGTTTAATCT GAAGAGACCT TGCTGCATGA AGAGGAAAAT GTTATCCTTG CATTTGGCAG ATGCTACTTC CTGGAGGTCC CAGAGTGTAA GAAACTGTTG CTTGGCATAA GTGCTGTCTG CGTTTCCCGG TCACCCTGGT GTGAGCCAGG GCACAGGCTC TTGGAGGCTT TATCGAGGCA GAGCTTCCCC AGTTGCAGGT GGGACCTCTC ACTTAGAAGT AAGCACAGCA AGTGGGAACT GCAGGGAGCC

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001145443, NM_001282630, NM_001314063, NM_001323016, NM_001323017, NM_004566, NR_136554, NM_001363545
• UniProt ID: Q16875
• Synonyms: iPFK-2; IPFK2; PFK2
• Summary: The protein encoded by this gene belongs to a family of bifunctional proteins that are involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate (F2,6BP), and a fructose-2,6-biphosphatase activity that catalyzes the degradation of F2,6BP. This protein is required for cell cycle progression and prevention of apoptosis. It functions as a regulator of cyclin-dependent kinase 1, linking glucose metabolism to cell proliferation and survival in tumor cells. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2016]