IL6 Human Gene Knockout Kit (CRISPR)

CAT#: KN202078

IL6 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: IL6
• Locus ID: 3569
• Components:

  KN202078G1, IL6 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: CGACCGCCGCTGGCAGTTCC

  KN202078G2, IL6 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: CCTTCTCCACAAGTAAGTGC

  KN202078D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  TGACTGGTAG TATTACCTTC TTCATAATCC CAGGCTTGGG GGGCTGCGAT GGAGTCAGAG GAAACTCAGT TCAGAACATC TTTGGTTTTT ACAAATACAA ATTAACTGGA ACGCTAAATT CTAGCCTGTT AATCTGGTCA CTGAAAAAAA ATTTTTTTTT TTTCAAAAAA CATAGCTTTA GCTTATTTTT TTTCTCTTTG TAAAACTTCG TGCATGACTT CAGCTTTACT CTTTGTCAAG ACATGCCAAA GTGCTGAGTC ACTAATAAAA GAAAAAAAGA AAGTAAAGGA AGAGTGGTTC TGCTTCTTAG CGCTAGCCTC AATGACGACC TAAGCTGCAC TTTTCCCCCT AGTTGTGTCT TGCCATGCTA AAGGACGTCA CATTGCACAA TCTTAATAAG GTTTCCAATC AGCCCCACCC GCTCTGGCCC CACCCTCACC CTCCAACAAA GATTTATCAA ATGTGGGATT TTCCCATGAG TCTCAATATT AGAGTCTCAA CCCCCAATAA ATATAGGACT GGAGATGTCT GAGGCTCATT CTGCCCTCGA GCCCACCGGG AACGAAAGAG AAGCTCTATC TCCCCTCCAG GAGCCCAGCT AGGCAGGCTC CCAGCTGTGC TGTCAGCTCA CCCCTGCGCT CGCTCCCCTC CGGCACAGGC GCCTTCGGTC CAGTTGCCTT CTCCCTGGGG CTGCTCCTGG TGTTGCCTGC TGCCTTCCCT GCCCCAGTAC CCCCAGGAGA AGATTCCAAA GATGTAGCCG CCCCACACAG ACAGCCACTC ACCTCTTCAG AACGAATTGA CAAACAAATT CGGTACATCC TCGACGGCAT CTCAGCCCTG AGAAAGGAGG TGGGTAGGCT TGGCGATGGG GTTGAAGGGC CCGGTGCGCA TGCGTTCCCC TTGCCCCTGC GTGTGGCCGG GGGCTGCCTG CATTAGGAGG TCTTTGCTGG GTTCTAGAGC ACTGTAGATT TGAGGCCAAC GGGGCCGACT AGACTGACTT CTGTATTTAT CCTTTGCTGG TGTCAGGAAG TTCCTTTCCT TTCTGGAAAA TGCAGAATGG GTCTGAAATC CATGCCCACC TTTGGCATGA GCTGAGGGTT ATTGCTTCTC AGGGCTTCCT TTTCCCTTTC CAAAAAATTA GGTCTGTGAA GCTCCTTTTT GTCCCCCGGG CTTTGGAAGG ACTAGAAAAG TGCCACCTGA

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_000600, NM_001318095
• UniProt ID: P05231
• Synonyms: BSF-2; BSF2; CDF; HGF; HSF; IFN-beta-2; IFNB2; IL-6
• Summary: This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). [provided by RefSeq, Aug 2020]