PML Protein (PML) Human Gene Knockout Kit (CRISPR)

CAT#: KN200700

PML - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: PML Protein
• Locus ID: 5371
• Components:

  KN200700G1, PML Protein gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: CTCGGAGATCGGGCGGGTGC

  KN200700G2, PML Protein gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: GGCCTTCAGAGGGGGTCTCG

  KN200700D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  CCAAAATTAG TTCCCAAACA GGCTTTTGCA AGAGCTATCT TTTCTGTTTC AAGTGTCCCC CGTACCCCAC CACCTACAAC CCTAAATCCC TGGTGAATTT CCACTGCTTT TGCAGGTCCC AGCTGTGGGC TCTCCTTTCC AGAATGTCTC CGTTTGCAGG CTTGCTCTTC CATTACATCT ACGGCTCTTA GAAAGCAGGG GCTGCGTGTG GCTCATCTTT GTACGCCCAG CTCCCGGCAG GACGTGGCAC GAAGCAGTGC CAGTGTGAAC GGATGAATGG ATCAAAGCCG GGAGCAGGGC TGCCCCCCTG CAGCTCTGCC CTACCTCTCC CGCTTTACCG TAAGTCAGCG GTAGGTCTGC AGCTCTCCGC CTCTACCTCC TCCCCGCTCT GGGCGTGTCT TTAAAACCCA CAGTCGGCCT CTCTGCCCCC TAGAACCGCC CCCAGCTTCT GTCTCACTTC CTCTCCAGAG GCGGGCCCTG AGCCGGCACC TCCCCTTTCG GACAGCTCAA GGGACTCAGC CAACTGGCTC ACGCCTCCCC TTCAGCTTCT CTTCACGCAC TCCAAGATCT AAACCGAGAA TCGAAACTAA GCTGGGGTCC TACAGAGGTA CTATTGGGTT AGGGGATGAT GGGGTTAAGC TTTGTTGGTT TGCTGTGGTG GGGAGAGGCG GGAAGAGAGG GTCTAACGGA GGATTTGGTC AAGTACCCTA GAGAGTGACA CAAAGCGGGA AGTCCAGACA CCAGGGTCCT GACCGTCTCG GGTGGGGCAG GGAAGGGAGG GTAGGATAGA GTAGAAAAGA GGACACGGAG GAGTTGGGGC GGCCTCGCTG GGCTGCGGTT TCTCCACTGA GCAGTTGGGC AAGGTGAGAA GGGTCAGTGG CCTCCGGGCC TGGGCCCTTC CGCCCACCCT CGAGCCCTGC CTCAACTTTG CCTCAGATGC AGGACTTCAG ATTAGGGAGG ATGGAGGTAG TACCCCTGTT GCGCTGGCCT GGAGCCAGGG GCATGTCCCA GGCACGGCAA AACTAAAACC AACTTCCCAG ATCCGAGGTG AGAAACTGGC TCAGACTGAA GAGGTATCTT TGCCAAGGCC TCCCAGCTCA TGTGGTTTCT GCTTAAGGAA GCCTCCCCAA CGAACCCTTC TCTTGCCACA CCTTTCCTGC CCACCTCCCA CCTCCCCCGA CAAAGGAACT ACTTGGGTTT

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_002675, NM_033238, NM_033239, NM_033240, NM_033242, NM_033244, NM_033245, NM_033246, NM_033247, NM_033249, NM_033250
• UniProt ID: P29590
• Synonyms: MYL; PP8675; RNF71; TRIM19
• Summary: The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This phosphoprotein localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. The gene is often involved in the translocation with the retinoic acid receptor alpha gene associated with acute promyelocytic leukemia (APL). Extensive alternative splicing of this gene results in several variations of the protein's central and C-terminal regions; all variants encode the same N-terminus. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]