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Anti-ALK TRUEMAB Antibody Clone 1A4
TrueMAB Antibodies - Made against Authentic Protein Antigens
|Human recombinant protein fragment corresponding to amino acids 1300-1620 of human ALK (NP_004295) produced in E.coli.|
|| WB 1:2000,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens anaplastic lymphoma receptor tyrosine kinase (ALK)|
Entrez Gene 238 Human
|This gene encodes a receptor tyrosine kinase, which belongs to the insulin receptor superfamily. This protein comprises an extracellular domain, an hydrophobic stretch corresponding to a single pass transmembrane region, and an intracellular kinase domain. It plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. This gene has been found to be rearranged, mutated, or amplified in a series of tumours including anaplastic large cell lymphomas, neuroblastoma, and non-small cell lung cancer. The chromosomal rearrangements are the most common genetic alterations in this gene, which result in creation of multiple fusion genes in tumourigenesis, including ALK (chromosome 2)/EML4 (chromosome 2), ALK/RANBP2 (chromosome 2), ALK/ATIC (chromosome 2), ALK/TFG (chromosome 3), ALK/NPM1 (chromosome 5), ALK/SQSTM1 (chromosome 5), ALK/KIF5B (chromosome 10), ALK/CLTC (chromosome 17), ALK/TPM4 (chromosome 19), and ALK/MSN (chromosome X).[provided by RefSeq, Jan 2011]. |
|Protein KinaseDruggable Genome |
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALK (RC222485, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALK.
Western blot analysis of extracts (35ug) from H2228 and A549 cell lines by using anti-ALK monoclonal antibody. (TA801287, 1:10,000)
Immunohistochemical staining of paraffin-embedded Human non-small cell lung cancer sample with ALK translocation detected by FISH using anti-ALK mouse monoclonal antibody. (TA801287, 1:50)
Immunohistochemical staining of paraffin-embedded Human large B cell lymphoma with ALK translocation using anti-ALK mouse monoclonal antibody. (TA801287, 1:50)
Immunohistochemical staining of paraffin-embedded ALK-positive lung tumor xenograft using anti-ALK mouse monoclonal antibody. (TA801287, 1:50)
Immunohistochemical staining of paraffin-embedded Human non-small cell lung cancer sample with EML4-ALK translocation detected by PCR using anti-ALK mouse monoclonal antibody. (TA801287, 1:50)
Immunohistochemistry staining of paraffin-embedded human cell line H1975 (upper left) and H2228 (upper right) on IHC antibody quality control slide using anti-ALK mouse monoclonal antibody TA801287 (1:400). The ALK rearrangement in H2228 cells is labeled with ALK Breakapart probe in FISH test (lower right, 60X) and the control of H1975 cell at the same FISH probe test (lower left, 60X).