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Anti-ATP6AP1 GAT Antibody Polyclonal
Generated by Genomic Immunization
Also for ATP6AP1 (NM_001183)
|DNA immunization. This antibody is specific for the Middle Region of the target protein.|
|| WB 1:20000, IHC 1:150,ELISA 1:100-1:2000
|20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0|
|The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
|This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT
|Homo sapiens ATPase, H+ transporting, lysosomal accessory protein 1 (ATP6AP1)|
|16A; Ac45; ATP6IP1; ATP6S1; CF2; VATPS1; XAP-3; XAP3|
|This gene encodes a component of a multisubunit enzyme (1 mDa MW) that mediates acidification of eukaryotic intracellular organelles. Vacuolar ATPase (V-ATPase) is comprised of a cytosolic V1 (site of the ATP catalytic site) and a transmembrane V0 domain. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, and receptor-mediated endocytosis. The encoded protein of this gene is approximately 45 kD and may assist in the V-ATPase-mediated acidification of neuroendocrine secretory granules.|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATP6AP1 (RC200394, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATP6AP1.
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-ATP6AP1 Rabbit Polyclonal antibody. (TA590072)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-ATP6AP1 Rabbit Polyclonal antibody. (TA590072)
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-ATP6AP1 Rabbit Polyclonal antibody. (TA590072)
Immunohistochemistry: ATP6AP1 Antibody - Most of the normal tissues displayed moderate to strong cytoplasmic positivity which was occasionally combined with membranous and nuclear staining. Malignant tissues generally displayed moderate cytoplasmic immunoreactivity. Many cases of malignant gliomas exhibited strong staining which also was observed in occasional other tumors. Image and statement courtesy of the Human Protein Atlas (HPA).