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Anti-MAPK3 TRUEMAB Antibody Clone OTI4D7

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA505700 MAPK3 (ERK1) mouse monoclonal antibody, clone OTI4D7 (formerly 4D7) 100ul $379 In Stock
LC400968 MAPK3 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF505700 Carrier-free (BSA/glycerol-free) MAPK3 mouse monoclonal antibody, clone OTI4D7 (formerly 4D7) 100ug $450 3 Days
Conjugation is available for this antibody: choose conjugation type
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WB(2)
IHC(5)
IF(1)
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Also for MAPK3 (NM_002746)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human MAPK3(NP_002737) produced in HEK293T cell.
Clone NameClone OTI4D7 IsotypeIgG1
Species ReactivityHuman , Dog , Monkey Concentration1 mg/ml
Guaranteed Application *WB, IHC, IF Suggested Dilutions WB 1:200~2000, IHC 1:150, IF 1:100,
Predicted MW Explanation 43 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens mitogen-activated protein kinase 3 (MAPK3), transcript variant 1
Alternative NameERK-1; ERK1; ERT2; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK; PRKM3
Database LinkNP_002737
Entrez Gene 5595 Human
Entrez Gene 708938 Monkey
Entrez Gene 100855877 Dog
FunctionThe protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq].
Related PathwayProtein KinaseES Cell Differentiation/IPSDruggable Genome MAPK signaling pathwayErbB signaling pathwayChemokine signaling pathwayOocyte meiosismTOR signaling pathwayVascular smooth muscle contractionMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK3 (RC204196, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK3.
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Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-MAPK3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MAPK3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505700)
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MAPK3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505700)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MAPK3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505700)
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Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-MAPK3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505700)
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Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-MAPK3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505700)
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Anti-MAPK3 mouse monoclonal antibody (TA505700) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAPK3(RC204196).
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