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Home Antibody All anti-PPP1R15A antibodies

Anti-PPP1R15A TRUEMAB Antibody clone 3D12

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA504310
  • PPP1R15A (GADD34) mouse monoclonal antibody, clone 3D12
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC415358) , 20ug Explanation
100ul
30ul
325 In Stock
CF504310
  • Carrier-free (BSA/glycerol-free) PPP1R15A mouse monoclonal antibody,clone 3D12
100ug $450 3-4 weeks
This antibody qualifies for Season of Giving promotion: Earn $50 Amazon gift card (use promo code SPQ12081) or donation to Cancer.org (promo code SPQ12082) with $600 purchase of UltraMAB or TrueMAB. Click for details
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WB(2)
IHC(6)
IF(1)
FC(1)
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Also for PPP1R15A (NM_014330)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human PPP1R15A(NP_055145) produced in HEK293T cell.
Clone Nameclone 3D12 IsotypeIgG2b
Species ReactivityHuman , Dog , Rat , Monkey , Mouse Concentration0.25 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested Dilutions WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100,
Predicted MW Explanation 73.3 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens protein phosphatase 1, regulatory subunit 15A (PPP1R15A)
Alternative NameGADD34
Database LinkNP_055145
Entrez Gene 23645 Human
Entrez Gene 17872 Mouse
Entrez Gene 171071 Rat
Entrez Gene 718350 Monkey
Entrez Gene 476419 Dog
FunctionThis gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The induction of this gene by ionizing radiation occurs in certain cell lines regardless of p53 status, and its protein response is correlated with apoptosis following ionizing radiation. [provided by RefSeq, Jul 2008].
Related PathwayDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPP1R15A (RC200581, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPP1R15A.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PPP1R15A monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
IHC Image
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-PPP1R15A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504310)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PPP1R15A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504310)
IHC Image
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-PPP1R15A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504310)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PPP1R15A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504310)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PPP1R15A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504310)
IHC Image
Immunohistochemical staining of paraffin-embedded Human tonsil using anti-PPP1R15A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504310)
IF Image
Anti-PPP1R15A mouse monoclonal antibody (TA504310) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PPP1R15A(RC200581).
FC Image
HEK293T cells transfected with either RC200581 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PPP1R15A antibody(TA504310), and then analyzed by flow cytometry.

 

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