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Home Antibody All anti-SMS antibodies

Anti-SMS TRUEMAB Antibody Clone OTI5F2

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA503098 SMS (Spermine synthase) mouse monoclonal antibody, clone OTI5F2 (formerly 5F2) 100ul $325 In Stock
LC417877 SMS HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF503098 Carrier-free (BSA/glycerol-free) SMS mouse monoclonal antibody, clone OTI5F2 (formerly 5F2) 100ug $450 3-4 weeks
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WB(2)
IHC(2)
IF(2)
FC(1)

OriGene Data

ImmunogenFull length human recombinant protein of human SMS (NP_004586) produced in HEK293T cell.
Clone NameClone OTI5F2 IsotypeIgG2b
Species ReactivityHuman Concentration0.59 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:2000, IHC 1:150, IF 1:50~100, FLOW 1:100,
Predicted MW Explanation 41.1 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens spermine synthase (SMS), transcript variant 1
Alternative NameMRSR; SPMSY; SpS; SRS
Database LinkNP_004586
Entrez Gene 6611 Human
FunctionThis gene encodes a protein belonging to the spermidine/spermin synthase family. Pseudogenes of this gene are located on chromosomes 1, 5, 6 and X. Mutations in this gene are associated with X-linked Snyder-Robinson mental retardation syndrome. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012].
Related Pathway Cysteine and methionine metabolismArginine and proline metabolismbeta-Alanine metabolismGlutathione metabolismMetabolic pathways

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SMS (RC200619, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SMS.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SMS monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)
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Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-SMS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503098)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-SMS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503098)
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Anti-SMS mouse monoclonal antibody (TA503098) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SMS(RC200619).
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Immunofluorescent staining of HeLa cells using anti-SMS mouse monoclonal antibody (TA503098).
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HEK293T cells transfected with either RC200619 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SMS antibody(TA503098), and then analyzed by flow cytometry.
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