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Anti-DCK TRUEMAB Antibody Clone OTI16G6

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA502698 DCK mouse monoclonal antibody, clone OTI16G6 (formerly 16G6) 100ul $379 In Stock
LC400272 DCK HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF502698 Carrier-free (BSA/glycerol-free) DCK mouse monoclonal antibody, clone OTI16G6 (formerly 16G6) 100ug $450 3-4 weeks
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WB(3)
IHC(5)
FC(2)

OriGene Data

ImmunogenFull length human recombinant protein of human DCK (NP_000779) produced in HEK293T cell.
Clone NameClone OTI16G6 IsotypeIgG1
Species ReactivityHuman Concentration0.49 mg/ml
Guaranteed Application *WB, IHC, FC Suggested Dilutions WB 1:500~2000, IHC 1:150, FLOW 1:100,
Predicted MW Explanation 30.3 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens deoxycytidine kinase (DCK)
Alternative NameMGC117410; MGC138632
Database LinkNP_000779
Entrez Gene 1633 Human
FunctionDeoxycytidine kinase (DCK) is required for the phosphorylation of several deoxyribonucleosides and their nucleoside analogs. Deficiency of DCK is associated with resistance to antiviral and anticancer chemotherapeutic agents. Conversely, increased deoxycytidine kinase activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. DCK is clinically important because of its relationship to drug resistance and sensitivity. [provided by RefSeq, Jul 2008].
Related PathwayDruggable Genome Purine metabolismPyrimidine metabolism

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DCK (RC210767, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DCK.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DCK monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-DCK monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-DCK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502698)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-DCK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502698)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-DCK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502698)
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Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-DCK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502698)
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Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-DCK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502698)
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Flow cytometric Analysis of Hela cells, using anti-DCK antibody(TA502698),(Red), compared to a nonspecific negative control antibody,(Blue).
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Flow cytometric Analysis of Jurkat cells, using anti-DCK antibody(TA502698),(Red), compared to a nonspecific negative control antibody,(Blue).
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