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Anti-AFP TRUEMAB Antibody Clone OTI2A9
TrueMAB Antibodies - Made against Authentic Protein Antigens
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|Full length human recombinant protein of human AFP (NP_001125) produced in HEK293T cell.|
|Human , Dog , Monkey
|WB, IHC, IF, FC
||WB 1:200~500, IHC 1:150, IF 1:100, FLOW 1:100
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens alpha-fetoprotein (AFP)|
|AFPD; FETA; HPAFP|
Entrez Gene 174 Human
Entrez Gene 704786 Monkey
Entrez Gene 403551 Dog
|This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq].|
|Secreted ProteinDruggable Genome |
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AFP (RC206622, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AFP.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-AFP monoclonal antibody.
Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-AFP monoclonal antibody.(1:200)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-AFP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501782)
Anti-AFP mouse monoclonal antibody (TA501782) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY AFP(RC206622).
HEK293T cells transfected with either RC206622 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-AFP antibody(TA501782), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-AFP antibody(TA501782),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).