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Anti-NUMB TRUEMAB Antibody Clone OTI1A7

TrueMAB™ Antibodies - Made against Authentic Protein Antigens


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA501614 NUMB mouse monoclonal antibody, clone OTI1A7 (formerly 1A7) 100ul 325
In Stock
LC401229 NUMB HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF501614 Carrier-free (BSA/glycerol-free) NUMB mouse monoclonal antibody, clone OTI1A7 (formerly 1A7) 100ug $450 3 Days
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OriGene Data

ImmunogenFull length human recombinant protein of human NUMB (NP_003735) produced in HEK293T cell.
Clone NameClone OTI1A7 IsotypeIgG1
Species ReactivityHuman Concentration0.6 mg/ml
Guaranteed Application *WB, IF, FC Suggested DilutionsWB 1:2000, IF 1:100, FLOW 1:100
Predicted MW Explanation 69.3 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens numb homolog (Drosophila) (NUMB), transcript variant 3
Alternative NameC14orf41; c14_5527; S171
Database LinkNP_003735
Entrez Gene 8650 Human
FunctionThe protein encoded by this gene plays a role in the determination of cell fates during development. The encoded protein, whose degradation is induced in a proteasome-dependent manner by MDM2, is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. Four transcript variants encoding different isoforms have been found for this gene.
Related Pathway Notch signaling pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NUMB (RC216230, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NUMB.

WB Image
Western blot analysis of extracts (10ug) from 3 different cell lines by using anti-NUMB monoclonal antibody.(1:200)

IF Image
Anti-NUMB mouse monoclonal antibody (TA501614) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NUMB(RC216230 ).

FC Image
HEK293T cells transfected with either RC216230 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-NUMB antibody(TA501614), and then analyzed by flow cytometry.


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