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Home Antibody All anti-PRKAR2A antibodies

Anti-PRKAR2A TRUEMAB Antibody Clone OTI5F1

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA501195 anti-PRKAR2A (PKA R2 ) mouse monoclonal antibody, clone OTI5F1 (formerly 5F1) 100ul
30ul
$379 In Stock
LC401337 PRKAR2A HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF501195 Carrier-free (BSA/glycerol-free) PRKAR2A mouse monoclonal antibody, clone OTI5F1 (formerly 5F1) 100ug $450 3-4 weeks
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WB(3)
IHC(1)
IF(1)
FC(1)
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Also for PRKAR2A (NM_004157)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human PRKAR2A (NP_004148) produced in HEK293T cell.
Clone NameClone OTI5F1 IsotypeIgG1
Species ReactivityHuman, Dog, Rat, Monkey Concentration0.57 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:1000~2000, IHC 1:50, IF 1:100, FLOW 1:100
Predicted MW Explanation 45.3 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens protein kinase, cAMP-dependent, regulatory, type II, alpha (PRKAR2A)
Alternative NamePKR2; PRKAR2
Database LinkNP_004148
Entrez Gene 5576 Human
Entrez Gene 29699 Rat
Entrez Gene 708872 Monkey
Entrez Gene 100685147 Dog
FunctioncAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of cAMP-dependent protein kinase. This subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER).
Related PathwayDruggable Genome ApoptosisInsulin signaling pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRKAR2A (RC220376, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRKAR2A.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PRKAR2A monoclonal antibody.
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Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-PRKAR2A monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
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Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PRKAR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501195, Dilution 1:50)
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Anti-PRKAR2A mouse monoclonal antibody (TA501195) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PRKAR2A(RC220376 ).
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HEK293T cells transfected with either RC220376 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PRKAR2A antibody(TA501195), and then analyzed by flow cytometry.
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