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Anti-BSG TRUEMAB Antibody Clone OTI9H5
TrueMAB Antibodies - Made against Authentic Protein Antigens
|Full length human recombinant protein of human BSG(NP_001719) produced in HEK293T cell.|
|Human, Dog, Rat, Monkey
|WB, IHC, IF, FC
||WB 1:1500~3000, IHC 1:50, IF 1:100, FLOW 1:100
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BSG (RC219464, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BSG.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BSG monoclonal antibody.
Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-BSG monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-BSG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501164, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-BSG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501164, Dilution 1:50)
Anti-BSG mouse monoclonal antibody (TA501164) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BSG(RC219464).
Immunofluorescent staining of HT29 cells using anti-BSG mouse monoclonal antibody (TA501164).
HEK293T cells transfected with either RC219464 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-BSG antibody(TA501164), and then analyzed by flow cytometry.