Home Antibody All anti-PKMYT1 antibodies
Anti-PKMYT1 TRUEMAB Antibody clone 2B4
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for PKMYT1 (NM_004203)
|Full length human recombinant protein of human PKMYT1 (NP_004194) produced in HEK293T cell.|
|WB, IF, FC
|| IHC 1:100~200, WB: 1:200
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens protein kinase, membrane associated tyrosine/threonine 1 (PKMYT1), transcript variant 1|
Entrez Gene 9088 Human
|The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase preferentially phosphorylates and inactivates cell division cycle 2 protein (CDC2), and thus negatively regulates cell cycle G2/M transition. This kinase is associated with the membrane throughout the cell cycle. Its activity is highly regulated during the cell cycle. Protein kinases AKT1/PKB and PLK (Polo-like kinase) have been shown to phosphorylate and regulate the activity of this kinase. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq]. |
|Protein KinaseDruggable Genome Cell cycleOocyte meiosisProgesterone-mediated oocyte maturation|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PKMYT1 (RC222657, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PKMYT1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PKMYT1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)
Anti-PKMYT1 mouse monoclonal antibody (TA500839) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PKMYT1(RC222657).
Flow cytometric Analysis of Hela cells, using anti-PKMYT1 antibody(TA500839),(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-PKMYT1 antibody(TA500839),(Red), compared to a nonspecific negative control antibody,(Blue).