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Anti-TCF7 Antibody EPR2035
Also for TCF7 (NM_001134851)
|A synthetic peptide corresponding to residues in human TCF-1 / TCF-7 was used as an immunogen.|
||Tissue culture supernatant
||Lot dependent; please refer to CoA along with shipment
||WB: 1:1000 - 1:1000; ICC/IF: 1:100 - 1:250
|Does not react with Rat. Is unsuitable for Flow Cyt,IHC-P or IP.|
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
|Is unsuitable for Flow Cyt,IHC-P or IP.
|Homo sapiens transcription factor 7 (T-cell specific, HMG-box) (TCF7), transcript variant 3|
|TCF-1/TCF-7 is a transcriptional activator involved in T-cell lymphocyte differentiation, and it is necessary for the survival of CD4+ CD8+ immature thymocytes. As a HMG box transcription factor family member, it binds to the T-lymphocyte-specific enhancer element (5'-WWCAAAG-3'). (1, 2). Expression of TCF-1/TCF-7 is limited to T cells and NK cells (2). It also acts directly downstream of the canonical Wnt–ß-catenin signaling pathway. Non-phosphorylated ß-catenin accumulates in the cytoplasm, translocates to the nucleus and together with TCF-1/TCF-7, induces expression of Wnt (3).|
Wnt Signaling Pathway
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Western blot - Anti-TCF7 antibody [EPR2035]; All lanes : Anti-TCF7 antibody [EPR2035] at 1/1000 dilution.Lane 1 : Mouse thymus lysate.Lane 2 : Fetal thymus lysate.Lane 3 : Jurkat cell lysate.Lysates/proteins at 10 µg per lane.Secondary.Goat ant-rabbit HRP conjugated antibody at 1/2000 dilution.Predicted band size : 42 kDa.Observed band size : 25-55 kDa .
Immunocytochemistry/ Immunofluorescence - Anti-TCF7 antibody [EPR2035]; Immunofluorescent staining of HeLa cells labelling TCF7 with TA311086 at 1/100.