Home Antibody All anti-NR3C1 antibodies
Anti-NR3C1 Antibody EPR4595
Also for NR3C1 (NM_000176)
|A recombinant protein fragment corresponding to amino acids 2-149 of human Glucocorticoid Receptor was used as an immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
||WB: 1:50000 - 1:200000; FC: 1:100 - 1:1000
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for ICC,IHC-P or IP.
* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.
Western blot - Glucocorticoid Receptor antibody [EPR4595]; All lanes : Anti-Glucocorticoid Receptor antibody [EPR4595] at 1/50000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : A431 cell lysate.Lane 3 : Jurkat cell lysate.Lane 4 : HepG2 cell lysate.Lysates/proteins at 10 ug per lane.Predicted band size : 86 kDa.
Flow Cytometry - Anti-Glucocorticoid Receptor antibody [EPR4595]; Overlay histogram showing Jurkat cells stained with TA311010 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.