OriGene Technologies, Inc.
Search:    
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-NR3C1 antibodies

Anti-NR3C1 Antibody EPR4595

div

Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA311010
  • Rabbit Monoclonal antibody against Glucocorticoid Receptor (NR3C1)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC424874) , 20ug Explanation
100ul 325 3-7 Days
Add to Shopping Cart
spacer
WB(1)
FC(1)

OriGene Data

ImmunogenA recombinant protein fragment corresponding to amino acids 2-149 of human Glucocorticoid Receptor was used as an immunogen.
Clone NameEPR4595 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, FC Suggested DilutionsWB: 1:50000 - 1:200000; FC: 1:100 - 1:1000
Predicted MW Explanation 86 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for ICC,IHC-P or IP.

Reference Data

Target NameHomo sapiens nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) (NR3C1), transcript variant 1
Alternative NameGCCR; GCR; GCRST; GR; GRL
Database LinkNP_000167
Entrez Gene 2908 Human
Entrez Gene 14815 Mouse
Entrez Gene 24413 Rat
FunctionGlucocorticoid Receptor can act as both a transcription factor and as a regulator of other transcription factors. This protein can also be found in heteromeric cytoplasmic complexes along with heat shock factors and immunophilins. It is typically found in the cytoplasm until it binds a ligand, which induces transport into the nucleus. Mutations in Glucocorticoid Receptor are a cause of glucocorticoid resistance, or cortisol, resistance (1).
Related PathwayNuclear Hormone ReceptorTranscription FactorsDruggable Genome Neuroactive ligand-receptor interaction

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Glucocorticoid Receptor antibody [EPR4595]; All lanes : Anti-Glucocorticoid Receptor antibody [EPR4595] at 1/50000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : A431 cell lysate.Lane 3 : Jurkat cell lysate.Lane 4 : HepG2 cell lysate.Lysates/proteins at 10 ug per lane.Predicted band size : 86 kDa.
FC Image
Flow Cytometry - Anti-Glucocorticoid Receptor antibody [EPR4595]; Overlay histogram showing Jurkat cells stained with TA311010 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

 

spacer
Inc 5000 Healthcare Company Copyright © 2014 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title