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Anti-NCL Antibody EPR7952
|A synthetic peptide corresponding to residues in human Nucleolin was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, ASSAY, IHC
||WB: 1:10000 - 1:50000; IHC-P: 1:250 - 1:500; ICC/IF: 1:100 - 1:250
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt or IP.
|Homo sapiens nucleolin (NCL)|
|Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Nucleolin induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. Nucleolin also plays a role in the process of transcriptional elongation (1). |
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Western blot - Anti-Nucleolin antibody [5698-1]; All lanes : Anti-Nucleolin antibody [EPR7952] at 1/10000 dilution.Lane 1 : Fetal brain lysate.Lane 2 : K562 cell lysate.Lane 3 : HuT-78 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.Goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 76 kDa.Observed band size : 100 kDa .
Other-Anti-Nucleolin antibody [EPR7952](ab129200); Equilibrium disassociation constant (KD)..
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin antibody [EPR7952]; ab129200, at 1/250 dilution, staining Nucleolin in paraffin-embedded Human colon tissue by Immunohistochemistry.