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Home Antibody All anti-MIB1 antibodies

Anti-MIB1 Antibody EPR2762(2)

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA310692
  • Rabbit Monoclonal antibody against MIB1
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC412329) , 20ug Explanation
100ul 325 3-7 Days
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WB(1)
IHC(2)
Assay(1)
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Also for MIB1 (NM_020774)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the N-terminus of human MIB1 was used as an immunogen.
Clone NameEPR2762(2) IsotypeTissue culture supernatant
Species ReactivityMouse, Human (Does not react with: Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, ASSAY, IHC Suggested DilutionsWB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:250 - 1:500; ICC: 1:100 - 1:250
BufferDoes not react with Rat. Is unsuitable for Flow Cyt.
Purification pH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
Note Is unsuitable for Flow Cyt.

Reference Data

Target NameHomo sapiens mindbomb E3 ubiquitin protein ligase 1 (MIB1)
Alternative NameDIP-1; DIP1; LVNC7; MIB; ZZANK2; ZZZ6
Database LinkNP_065825
Entrez Gene 57534 Human
Entrez Gene 225164 Mouse
FunctionMIB1 is an E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. It positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. MIB1 mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis (1).
Related PathwayDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Anti-Mib1/Mindbomb antibody [EPR2762(2)]; All lanes : Anti-Mib1/Mindbomb antibody [EPR2762(2)] at 1/1000 dilution.Lane 1 : K562 cell lysates.Lane 2 : HeLa cell lysates.Lane 3 : Caco2 cell lysates.Lane 4 : NCCIT cell lysates.Lane 5 : Human brain lysates.Lysates/proteins at 10 ug per lane.Secondary.HRP-conjugated goat anti-rabbit at 1/2000 dilution.Predicted band size : 110 kDa.
Assay Image
Other-Anti-Mib1/Mindbomb antibody [EPR2762(2)](TA310692); Equilibrium disassociation constant (KD)..
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mib1/Mindbomb antibody [EPR2762(2)]; TA310692, at 1/250 dilution, staining Mib1/Mindbomb in formalin-fixed, paraffin-embedded Human kidney tissue by immunohistochemistry.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mib1/Mindbomb antibody [EPR2762(2)]; Immunohistochemical analysis of Human breast cancer tissue, staining Mib1/Mindbomb with TA310692.Tissue was fixed with paraformaldehyde and permeabilized with Tween buffer for 30 minutes at 20°C; antigen retrieval was by heat mediation in Tris/EDTA buffer (pH 9). Samples were incubated with primary antibody (1/5000 in diluent) for 30 minutes at 20°C. An undiluted HRP-conjugated goat anti-rabbit polyclonal IgG was used as the secondary antibody.

 

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