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Anti-MIB1 Antibody EPR2762(2)
Also for MIB1 (NM_020774)
|A synthetic peptide corresponding to residues near the N-terminus of human MIB1 was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
|WB, ASSAY, IHC
||WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:250 - 1:500; ICC: 1:100 - 1:250
|Does not react with Rat. Is unsuitable for Flow Cyt.|
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt.
|Homo sapiens mindbomb E3 ubiquitin protein ligase 1 (MIB1)|
|DIP-1; DIP1; LVNC7; MIB; ZZANK2; ZZZ6|
|MIB1 is an E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. It positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. MIB1 mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis (1). |
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Western blot - Anti-Mib1/Mindbomb antibody [EPR2762(2)]; All lanes : Anti-Mib1/Mindbomb antibody [EPR2762(2)] at 1/1000 dilution.Lane 1 : K562 cell lysates.Lane 2 : HeLa cell lysates.Lane 3 : Caco2 cell lysates.Lane 4 : NCCIT cell lysates.Lane 5 : Human brain lysates.Lysates/proteins at 10 µg per lane.Secondary.HRP-conjugated goat anti-rabbit at 1/2000 dilution.Predicted band size : 110 kDa.
Other-Anti-Mib1/Mindbomb antibody [EPR2762(2)](ab124929); Equilibrium disassociation constant (KD)..
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mib1/Mindbomb antibody [EPR2762(2)]; ab124929, at 1/250 dilution, staining Mib1/Mindbomb in formalin-fixed, paraffin-embedded Human kidney tissue by immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mib1/Mindbomb antibody [EPR2762(2)]; Immunohistochemical analysis of Human breast cancer tissue, staining Mib1/Mindbomb with ab124929.Tissue was fixed with paraformaldehyde and permeabilized with Tween buffer for 30 minutes at 20°C; antigen retrieval was by heat mediation in Tris/EDTA buffer (pH 9). Samples were incubated with primary antibody (1/5000 in diluent) for 30 minutes at 20°C. An undiluted HRP-conjugated goat anti-rabbit polyclonal IgG was used as the secondary antibody.