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Anti-MGEA5 Antibody EPR7154(B)


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SKU Description Amount Price Availability*  
TA310690 Rabbit Monoclonal antibody against MGEA5 100ul $325 In Stock
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OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human MGEA5 was used as an immunogen.
Clone NameEPR7154(B) IsotypeIgG
Species ReactivityMouse, Human (Does not react with: Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, Assay, IHC Suggested DilutionsWB: 1:10000 - 1:50000; IP: 1:100 - 1:500; IHC-P: 1:100 - 1:250
BufferpH: 7.20; Preservative: 0.01% Sodium azide; Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Note Is unsuitable for Flow Cyt or ICC.

Reference Data

Target NameHomo sapiens meningioma expressed antigen 5 (hyaluronidase) (MGEA5), transcript variant 2
Alternative NameMEA5; NCOAT; OGA
Database LinkNP_001135906
Entrez Gene 10724 Human
Entrez Gene 76055 Mouse
FunctionThe dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) addition and removal on serine and threonine residues is catalyzed by OGT, which adds O-GlcNAc, and MGEA5, a glycosidase that removes O-GlcNAc modifications (1). MGEA5 also possesses hyaluronidase activity (2).
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Anti-MGEA5 antibody [EPR7154(B)]; All lanes : Anti-MGEA5 antibody [EPR7154(B)] at 1/10000 dilution.Lane 1 : HeLa lysate.Lane 2 : BxPC-3 lysate.Lane 3 : JAR lysate.Lane 4 : Fetal brain lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 102 kDa.Observed band size : 130 kDa .

Assay Image
Other-Anti-MGEA5 antibody [EPR7154(B)](TA310690); Equilibrium disassociation constant (KD)..

IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGEA5 antibody [EPR7154(B)]; TA310690, at 1/100 dilution, staining MGEA5 in paraffin-embedded Human kidney tissue by Immunohistochemistry.


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