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Home Antibody All anti-GLI1 antibodies

Anti-GLI1 Antibody EPR4523

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SKU Description Amount Price Availability*  
TA310536 Rabbit Monoclonal antibody against Gli-1 (GLI1) 100ul $325 In Stock
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WB(1)
IHC(2)
IF(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human Gli-1 was used as an immunogen.
Clone NameEPR4523 IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:1000 - 1:10000; ICC/IF: 1:250 - 1:500; IHC-P: 1:250 - 1:500
BufferPreservative: 0.01% Sodium azide; Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
Purification Protein A purified
Note Is unsuitable for Flow Cyt or IP.

Reference Data

Target NameHomo sapiens GLI family zinc finger 1 (GLI1), transcript variant 2
Alternative NameGLI
Database LinkNP_001153517
Entrez Gene 2735 Human
FunctionGli-1 is a member of the Kruppel family of zinc finger proteins. This transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop (1).
Related PathwayStem cell relevant signaling - DSL/Notch pathwayES Cell Differentiation/IPSAdult stem cellsCancer stem cellsEmbryonic stem cellsTranscription FactorsDruggable Genome Hedgehog signaling pathwayPathways in cancerBasal cell carcinoma

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - Anti-Gli1 antibody [EPR4523]; All lanes : Anti-Gli1 antibody [EPR4523] at 1/1000 dilution.Lane 1 : RMS13 cell lysate.Lane 2 : A673 cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP-conjugated goat anti-rabbit IgG polyclonal at 1/2000 dilution.Predicted band size : 118 kDa.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gli1 antibody [EPR4523]; Immunohistochemical analysis of paraffin-embedded Human brain tissue labelling Gli1 with TA310536 at 1/250 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gli1 antibody [EPR4523]; Immunohistochemical analysis of paraffin-embedded Human testis tissue labelling Gli1 with TA310536 at 1/250 dilution.
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Immunocytochemistry/ Immunofluorescence - Anti-Gli1 antibody [EPR4523]; Immunofluorescent analysis of A673 cells labelling Gli1 with TA310536 at 1/250 dilution.
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