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Home Antibody All anti-GLI1 antibodies

Anti-GLI1 Antibody EPR4523

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA310536
  • Rabbit Monoclonal antibody against Gli-1 (GLI1)
100ul 325 In Stock
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WB(1)
IHC(2)
IF(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human Gli-1 was used as an immunogen.
Clone NameEPR4523 IsotypeTissue culture supernatant
Species ReactivityHuman ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:1000 - 1:10000; ICC/IF: 1:250 - 1:500; IHC-P: 1:250 - 1:500
BufferDoes not react with Mouse, Rat. Is unsuitable for Flow Cyt or IP.
Purification Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
Note Is unsuitable for Flow Cyt or IP.

Reference Data

Target NameHomo sapiens GLI family zinc finger 1 (GLI1), transcript variant 2
Alternative NameGLI
Database LinkNP_001153517
FunctionGli-1 is a member of the Kruppel family of zinc finger proteins. This transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop (1).
Related Pathway
Hedgehog signaling pathway

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WB Image
Western blot - Anti-Gli1 antibody [EPR4523]; All lanes : Anti-Gli1 antibody [EPR4523] at 1/1000 dilution.Lane 1 : RMS13 cell lysate.Lane 2 : A673 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP-conjugated goat anti-rabbit IgG polyclonal at 1/2000 dilution.Predicted band size : 118 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gli1 antibody [EPR4523]; Immunohistochemical analysis of paraffin-embedded Human brain tissue labelling Gli1 with TA310536 at 1/250 dilution.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gli1 antibody [EPR4523]; Immunohistochemical analysis of paraffin-embedded Human testis tissue labelling Gli1 with TA310536 at 1/250 dilution.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-Gli1 antibody [EPR4523]; Immunofluorescent analysis of A673 cells labelling Gli1 with TA310536 at 1/250 dilution.

 

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