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Anti-GLI1 Antibody EPR4523
Also for GLI1 (NM_001160045)
|A synthetic peptide corresponding to residues in human Gli-1 was used as an immunogen.|
||Tissue culture supernatant
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF
||WB: 1:1000 - 1:10000; ICC/IF: 1:250 - 1:500; IHC-P: 1:250 - 1:500
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt or IP.|
|Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
|Is unsuitable for Flow Cyt or IP.
|Homo sapiens GLI family zinc finger 1 (GLI1), transcript variant 2|
|Gli-1 is a member of the Kruppel family of zinc finger proteins. This transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop (1). |
Hedgehog signaling pathway
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Western blot - Anti-Gli1 antibody [EPR4523]; All lanes : Anti-Gli1 antibody [EPR4523] at 1/1000 dilution.Lane 1 : RMS13 cell lysate.Lane 2 : A673 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP-conjugated goat anti-rabbit IgG polyclonal at 1/2000 dilution.Predicted band size : 118 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gli1 antibody [EPR4523]; Immunohistochemical analysis of paraffin-embedded Human brain tissue labelling Gli1 with TA310536 at 1/250 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gli1 antibody [EPR4523]; Immunohistochemical analysis of paraffin-embedded Human testis tissue labelling Gli1 with TA310536 at 1/250 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-Gli1 antibody [EPR4523]; Immunofluorescent analysis of A673 cells labelling Gli1 with TA310536 at 1/250 dilution.