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Anti-CTSL1 Antibody EPR8011
Also for CTSL1 (NM_145918)
|A synthetic peptide corresponding to residues in human Cathepsin L1 was used as an immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, ASSAY, IHC, IF
||ICC/IF: Use at an assay dependent concentration; WB: 1:1000 - 1:10000; IHC-P: 1:50 - 1:100
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
|Tissue culture supernatant
|Is unsuitable for Flow Cyt,ICC or IP.
|Homo sapiens cathepsin L (CTSL), transcript variant 2|
|CATL; CTSL1; MEP|
Entrez Gene 1514 Human
Entrez Gene 13039 Mouse
Entrez Gene 25697 Rat
|Cathepsin L1 is a lysosomal cysteine proteinase that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. Cathepsin L1 has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. This protein, which is a member of the peptidase C1 family, is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor (1). |
|ProteaseDruggable Genome LysosomeAntigen processing and presentation|
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Western blot - Anti-Cathepsin L antibody [EPR8011]; All lanes : Anti-Cathepsin L antibody [EPR8011] at 1/1000 dilution.Lane 1 : A549 cell lysate.Lane 2 : HCT-116 cell lysate.Lane 3 : NIH/3T3 cell lysate.Lane 4 : PC-12 cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 38 kDa.
Western blot - Anti-Cathepsin L antibody [EPR8011]; Anti-Cathepsin L antibody [EPR8011] at 1/1000 dilution + HepG2 cell lysate at 10 ug.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 38 kDa.
Other-Anti-Cathepsin L antibody [EPR8011](TA310377); Equilibrium disassociation constant (KD)..
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin L antibody [EPR8011]; Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling Cathepsin L with TA310377 at 1/50 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin L antibody [EPR8011]; TA310377 stainingCathepsin LinHuman HepaRGcell by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed withformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 1%milk for 30 minutes at room temperature. Samples were incubated with primary antibody (1/1000) for30 minutes. AnAlexa Fluor?488-conjugated Donkey anti-rabbit IgG polyclonal (1/400) was used as the secondary antibody.