OriGene Technologies, Inc.
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-CD34 antibodies

Anti-CD34 Antibody EP373Y

div

Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA310274
  • Rabbit Monoclonal antibody against CD34
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC422593) , 20ug Explanation
100ul 325 In Stock
Add to Shopping Cart
WB(1)
IHC(5)
IF(1)
spacer
Also for CD34 (NM_001025109)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to C-terminal of human CD34 was used as an immunogen
Clone NameEP373Y IsotypeIgG
Species ReactivityMouse, Sheep, Dog, Human, Pig, Loxodonta Africana (African bush elephant) (Does not react with: Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:500; FC: 1:40;
BufferStore at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.

Reference Data

Target NameHomo sapiens CD34 molecule (CD34), transcript variant 1
Alternative NameCD34 antigen; OTTHUMP00000034733; OTTHUMP00000034734; hematopoietic progenitor cell antigen CD34; CD34 molecule
Database LinkNP_001020280
Entrez Gene 947 Human
Entrez Gene 12490 Mouse
Entrez Gene 415130 Dog
FunctionCD34 (Cluster of differentiation 34) is a single-pass type I transmembrane glycoprotein which primary functions as a cell to cell adhesion factor. As an adhesion factor, CD34 is expressed during stem/progenitor stage of lymphohematopoietic development and possibly mediates the stem cell attachment to the bone marrow ECM or stromal cells. CD34 is considered to be ideal marker for identifying and quantifying hematopoietic progenitor stem cells. With interaction with CrkL, CD34 becomes a substrate for PKC and CD34 surface expression is associated with activation of PKC.

*Mouse reactivity was determined by IHC on mouse tissues.
Related Pathway
Hematopoietic cell lineage

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Anti-CD34 antibody [EP373Y] (TA310274) at 1/100000 dilution + TF-1 at 10 ug. Secondary: HRP goat anti-rabbit at 1/2000 dilution
IHC Image
TA310274, at 1/100-1/250 dilution, staining human angiosarcoma by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
IHC Image
TA310274 showing positive staining in Normal tonsil vessels tissue.
IHC Image
TA310274 showing positive staining in Normal kidney vessels tissue.
IHC Image
TA310274 showing positive staining in Normal uterus vessels tissue.
IHC Image
IHC image of CD34 staining in Mouse normal brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with TA310274, 1/250 dilution, for 15 mins at room temperature. A Goat anti-Rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF Image
TA310274 staining CD34 in human embryonic stem cell-derived endothelial cells by Immunocytochemistry/ Immunofluorescence. Cell were fixed with 4% paraformaldehyde, permeabilized with 0.2% Triton X-100, and labeled with the primary antibody at a 1/200 dilution. An Alexa 647-conjugated secondary antibody was used at a 1/400 dilution. DAPI (blue) was used for nuclear staining.

 

spacer
Inc 5000 Healthcare Company Copyright © 2014 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title