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Anti-ABCA1 PHOSPHO Antibody EPR2485
Also for ABCA1 (NM_005502)
|A phospho specific peptide corresponding to residues surrounding serine 2054 of human ABCA1 was used as an immunogen. This antibody only detects ABCA1 phosphorylated at serine 2054.|
||Lot dependent; please refer to CoA along with shipment
||WB: 1:50000 - 1:200000; ICC/IF: 1:500 - 1:1000
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for Flow Cyt or IHC-P.
|Homo sapiens ATP-binding cassette, sub-family A (ABC1), member 1 (ABCA1)|
|ABC-1; ABC1; CERP; HDLDT1; TGD|
|ABCA1 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes, and are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. With cholesterol as its substrate, ABCA1 functions as a cholesterol efflux pump in the cellular lipid removal pathway. Mutations in this protein have been associated with Tangier's disease and familial high-density lipoprotein deficiency (1). It has been shown that Ser-1042 and Ser-2054, located in the nucleotide binding domains of ABCA1, are major phosphorylation sites for PKA. Phosphorylation of Ser-2054 within the nucleotide binding domain 2 was found to be particularly important for ApoA-I-dependent phospholipid efflux activity mediated by ABCA1 (2). |
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Western blot - Anti-ABCA1 (phospho S2054) antibody [EPR2485]; All lanes : Anti-ABCA1 (phospho S2054) [EPR2485] antibody at 1/50000 dilution.Lane 1 : SH-SY5Y cell lysate, untreated.Lane 2 : SH-SY5Y cell lysate, Okadaic Acid and Calyculin A treated.Lysates/proteins at 10 µg per lane.Secondary.Goat anti-Rabbit HRP at 1/2000 dilution.developed using the ECL technique.Predicted band size : 254 kDa.
Immunocytochemistry/ Immunofluorescence - Anti-ABCA1 (phospho S2054) antibody [EPR2485]; TA310159, at a 1/500 dilution, staining ABCA1 (green) in Okadaic Acid and Calyculin A treated SH-SY5Y cells by immunofluorescence. DNA is marked in blue.