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Anti-ACVR1B Antibody EPR4815
Also for ACVR1B (NM_004302)
|A synthetic peptide corresponding to residues in the extracellular domain of human ACVR1B was used as an immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF, FC
||WB: 1:1000 - 1:10000; IHC-P: 1:500 - 1:1000; FC: 1:100 - 1:500; ICC/IF: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for IP.
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Western blot - Activin A Receptor Type IB antibody [EPR4815]; All lanes : Anti-Activin A Receptor Type IB antibody [EPR4815] at 1/1000 dilution.Lane 1 : Human brain tissue lysate.Lane 2 : Human fetal kidney.Lane 3 : SH-SY5Y cell lysate.Lane 4 : U87-MG cell lysate.Lane 5 : Human fetal liver.Lysates/proteins at 10 ug per lane.Predicted band size : 57 kDa.Observed band size : 52 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Activin A Receptor Type IB antibody [EPR4815]; TA307816, at 1/500 dilution, staining Activin A receptor type IB in Human kidney tissue by immunohistochemistry.
Immunocytochemistry - Anti-Activin A Receptor Type IB antibody [EPR4815]; Alexa Fluor 488 goat anti-rabbit IgG was used as the secondary antibody. The cells (SH-SY5Y) were fixed with 2% paraformaldehyde for 20 min.
Flow Cytometry - Anti-Activin A Receptor Type IB antibody [EPR4815]; Overlay histogram showing HEK293 cells stained with TA307816 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.